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15-0595-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1128950 [Map]; Strand: c
      • AT5G04130.1 - [+] show detail - DNA GYRASE B2
        Distance: -859; Region: promoter
      • AT5G04130.2 - [+] show detail - DNA GYRASE B2
        Distance: -859; Region: promoter
      • AT5G04140.1 - [+] show detail - glutamate synthase 1
        Distance: -902; Region: promoter
    Flanking Genome Sequence: 15-0595-1 - 5-prime edge
    GGTTGAGGGG TGTGTGTGTG GCTGAAATAG ACTAGAGAAG CCTTAAGGAT 
    ATGAAATGTC CTTAAAGTTG CTCTAAAAGC CACCTTCTCT CTATCATATT
    CTCGTTGCCT ACTCTCTGTC ACTTATGGAA TTATGATGAT AAGATAAGAG
    CTACAGCTTA TCGTCAGTTG GCGTATCACG AGACGACCAC CCAAAAAAGA
    GCAAACACAA GCACCAAAGC GAACAGAAAT TAGAAATGAG ACGACACTTG
    TTGAAATATA GTCTGATAAA AATCTTTGTT ATTGTTTTGG ATC
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1129066 [Map]; Strand: c
      • AT5G04130.1 - [+] show detail - DNA GYRASE B2
        Distance: -975; Region: promoter
      • AT5G04130.2 - [+] show detail - DNA GYRASE B2
        Distance: -975; Region: promoter
      • AT5G04140.1 - [+] show detail - glutamate synthase 1
        Distance: -786; Region: promoter
      • AT5G04140.2 - [+] show detail - glutamate synthase 1
        Distance: -965; Region: promoter
    Flanking Genome Sequence: 15-0595-1 - 3-prime edge
    CCTCAACCCC ATACCCGTAC GTGTCTTTTC GTAAATTCAC ATATTTTTGT 
    TTAGTTATAC GTGTGTGTAT AAATATTTCT CATTAATATT AAATACCAAA
    ATCTCAACTA TCTAAAGAAA AAGTTGACCA GAATAAACAA TGGATACTAT
    TCCAAACTTG ACACATTTAT AAAGTCACCA CTACAAAAAA TGTATTGATC
    GTAATCACTA TGTATGAATA AATATTTTTT GTATTTTTTT TTGGATTCAA
    TCATTTTTAC TATAATTTTC ATAATTTTAA AATCATTTGG TAAAACATTA
    TAATTTTTTT GTTANATTTG ATAGTTGATC TCTTATACTA CTAATTCCGA
    TATTTTTTCT TCAAAGTTTG GCGATATGTG AAAATGTCAT TTTCTATTTT
    GTTTATAAAT AATAACAACC TTTCAAAACA TAAATAGATG AAAATTGTNT
    AAATTAAAAT CCATGGTTTG TTCGGTTAAT AAAAATGTNT GAATAAATTA
    CAAACCTGAC TATTTTATGT ATCCTTTATC TGGGGGATAT GAATTTTAGG
    AGTAGTCACT ATCTTCCCTC TATCAAAATG ACAATTTATC TACCCTTAAT
    TTCATGTCTC AATCCGGTTT AATGACCAAA AGTGTNGTAT CAAACCGGTT
    AATCAACCGA ATTACCANCC CGGGCCGNNG ACCACNCNGN CCCNTNNA

AT5G04130.1

Model type
Protein coding
Short Description
DNA GYRASE B2
Computational Description
DNA GYRASE B2 (GYRB2); FUNCTIONS IN: DNA topoisomerase activity, DNA topoisomerase (ATP-hydrolyzing) activity, DNA binding, ATP binding; INVOLVED IN: DNA topological change, DNA metabolic process; LOCATED IN: mitochondrion; CONTAINS InterPro DOMAIN/s: DNA topoisomerase, type IIA, conserved site (InterPro:IPR018522), DNA topoisomerase, type IIA, subunit B (InterPro:IPR000565), DNA topoisomerase, type IIA, subunit B, domain 2 (InterPro:IPR013506), ATPase-like, ATP-binding domain (InterPro:IPR003594), DNA topoisomerase, type IIA, subunit B, C-terminal (InterPro:IPR002288), DNA topoisomerase, type IIA, subunit B/N-terminal, alpha-beta (InterPro:IPR013759), DNA topoisomerase, type IIA, subunit B/N-terminal (InterPro:IPR001241), Ribosomal protein S5 domain 2-type fold (InterPro:IPR020568), Toprim domain (InterPro:IPR006171), Ribosomal protein S5 domain 2-type fold, subgroup (InterPro:IPR014721), DNA topoisomerase, type IIA, central (InterPro:IPR013760); BEST Arabidopsis thaliana protein match is: DNA GYRASE B1 (TAIR:AT3G10270.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G04130.2

Model type
Protein coding
Short Description
DNA GYRASE B2
Computational Description
DNA GYRASE B2; FUNCTIONS IN: DNA topoisomerase activity, DNA topoisomerase (ATP-hydrolyzing) activity, DNA binding, ATP binding; INVOLVED IN: DNA topological change, DNA metabolic process; LOCATED IN: mitochondrion; CONTAINS InterPro DOMAIN/s: Ribosomal protein S5 domain 2-type fold (InterPro:IPR020568), DNA topoisomerase, type IIA, subunit B, domain 2 (InterPro:IPR013506), Ribosomal protein S5 domain 2-type fold, subgroup (InterPro:IPR014721), ATPase-like, ATP-binding domain (InterPro:IPR003594), DNA topoisomerase, type IIA, subunit B (InterPro:IPR000565), DNA topoisomerase, type IIA, subunit B/N-terminal (InterPro:IPR001241); BEST Arabidopsis thaliana protein match is: DNA GYRASE B1 (TAIR:AT3G10270.1); Has 25944 Blast hits to 25881 proteins in 6578 species: Archae - 105; Bacteria - 19082; Metazoa - 171; Fungi - 228; Plants - 112; Viruses - 77; Other Eukaryotes - 6169 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G04140.1

Model type
Protein coding
Short Description
glutamate synthase 1
Curator Summary
Encodes a gene whose sequence is similar to ferredoxin dependent glutamate synthase (Fd-GOGAT). Expression in leaves is induced by light and sucrose. Proposed to be involved in photorespiration and nitrogen assimilation.
Computational Description
glutamate synthase 1 (GLU1); FUNCTIONS IN: protein binding, glutamate synthase (ferredoxin) activity; INVOLVED IN: positive regulation of glycine hydroxymethyltransferase activity, response to light stimulus, photorespiration; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase, class-II (InterPro:IPR000583), Aldolase-type TIM barrel (InterPro:IPR013785), Glutamate synthase, alpha subunit, C-terminal (InterPro:IPR002489), Glutamate synthase, central-N (InterPro:IPR006982), Glutamate synthase, central-C (InterPro:IPR002932), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: glutamate synthase 2 (TAIR:AT2G41220.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G04140.2

Model type
Protein coding
Short Description
glutamate synthase 1
Curator Summary
Encodes a gene whose sequence is similar to ferredoxin dependent glutamate synthase (Fd-GOGAT). Expression in leaves is induced by light and sucrose. Proposed to be involved in photorespiration and nitrogen assimilation.
Computational Description
glutamate synthase 1 (GLU1); FUNCTIONS IN: protein binding, glutamate synthase (ferredoxin) activity; INVOLVED IN: response to light stimulus, photorespiration; LOCATED IN: apoplast, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase, class-II (InterPro:IPR000583), Aldolase-type TIM barrel (InterPro:IPR013785), Glutamate synthase, alpha subunit, C-terminal (InterPro:IPR002489), Glutamate synthase, central-N (InterPro:IPR006982), Glutamate synthase, central-C (InterPro:IPR002932), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: glutamate synthase 2 (TAIR:AT2G41220.1); Has 17415 Blast hits to 17302 proteins in 2024 species: Archae - 267; Bacteria - 5686; Metazoa - 111; Fungi - 160; Plants - 179; Viruses - 0; Other Eukaryotes - 11012 (source: NCBI BLink).
Link
InterPro Scan - TAIR