1. Home
  2. Mutant Lines
  3. 15-4636-1

15-4636-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 17337149 [Map]; Strand: w
      • AT1G47290.1 - [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
        Distance: 0; Region: intron5 (CDS)
      • AT1G47290.2 - [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
        Distance: 0; Region: intron5 (CDS)
    Flanking Genome Sequence: 15-4636-1 - 3-prime edge 
    GAAAACGGTA GAGGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCACT 
    GTAATGGAAG TAGCAAGACT GATTAACATC ACTAAAAAAG GAGAGACCAG 
    AGCATAATAG GTCACAAGGA AAATAGATAG CATTTAGAAG GCCAAAGAGA 
    TAAAACAAAG AAAATAGAGG CAACCTTGGA TTTCCCAGCC CTGGCAGCAG 
    TAACAAGCGA TGGAACCATT AATTTATCAC CAGGACCAAA TATGCNTGCT 
    AGGACGTATG CAACAAGTGA GTAGTCCACT TCTTCCATTC GCTTTCAAAA 
    TCAAAGCT

AT1G47290.1

Model type
Protein coding
Short Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Curator Summary
Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Computational Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (3BETAHSD/D1); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (TAIR:AT2G26260.1); Has 24441 Blast hits to 24428 proteins in 2873 species: Archae - 619; Bacteria - 15419; Metazoa - 693; Fungi - 529; Plants - 1563; Viruses - 132; Other Eukaryotes - 5486 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G47290.2

Model type
Protein coding
Short Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
Curator Summary
Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
Computational Description
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (3BETAHSD/D1); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (TAIR:AT2G26260.1); Has 24445 Blast hits to 24432 proteins in 2873 species: Archae - 619; Bacteria - 15430; Metazoa - 691; Fungi - 529; Plants - 1560; Viruses - 132; Other Eukaryotes - 5484 (source: NCBI BLink).
Link
InterPro Scan - TAIR