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52-0116-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6833673 [Map]; Strand: c
      • AT5G20250.1 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.2 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.3 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.4 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
    Flanking Genome Sequence: 52-0116-1 - 5-prime edge
    ACTCTGTGAC GCCATTTTTG TTTCTCTCTC TCTCTCTTTG GCTTTCCTCG 
    TTTGGNGAGT TTTTTTACAA GTTTGTANGA CGCTTAACTC AGTTGAATTT
    ATAATATTAA ATAAATACNT TTCTTATTTT GGATTTTGTT TTTCTCATAA
    AGGATAAAAA AATTTGGATG TTGAATTGGA TCTCTCTTTT ATTTTCTAAT
    TACTTTGGTT TTTAAGTNAC ACGTCTTACG TTTCTTGNGA TTGTTTCTTT
    CGTATTGNGC AAGATTGCAA GTAATCTCAN ACATTAATTG ATATATTTTT
    CTATGGTTTA TGTATTAAAT GGTCAAATTA TGCAAAAATG TTGAGGTGAT
    TATGAATTTT GCGAAAGATG TGACATGACT ACTCAAATGA ATGAAAGATT
    AATACCACAA AAAGAAATAA AACAATAGTT GACCGTTGAC AAGTTTTAAA
    GGGAGGATGA GGATGCCACG TANGATAAAT GCTGGAACAC ATAAGGATAT
    TTAAAGATAT TNTGTCTTTT AAGGATTAAA AGATTTGTTN CCACGACCGT
    ATTACGTGTC ATCGTGTCAG CGGGTAATTG GGACGACCTT ATCACCAACT
    AAATTGTTTT CTTCACATCA ATCTCAAAAT ANAAGTTTTC TTGCAACTTG
    TANGCTAAAA TTGACTGTTT TTACCTTTAA TGATTTAATT GTTTTCTTAG
    NCCAATATTT GAAAAACTTT CCTG
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6833789 [Map]; Strand: c
      • AT5G20250.1 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.2 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.3 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (5UTR)
      • AT5G20250.4 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 52-0116-1 - 3-prime edge
    CACAGAGTTG CTACAATGGA ATCCATCGTA TAAAAAGGGT TCATCCTCTG 
    ATTCACTCTC TCACATTTCA TCGATTCTCC TCATCTTCTC TTCCATTCAA
    ATCTCACATT CCCTCTTCAC AACAACAGCT TCGTATTCGC TCATCTTCTT
    CGCTTTCTGG TATCGCATCT TAATCACCAT CTATCTCCTT TCAATCATTC
    TATGTTCCCA GATTTTGCGT CTAACAACGA CTTTTTATTG TTTTATCTCC
    ATATCTTGGA AATCGCAGGC ATTGTTCGTC TACTCGAGGA TTTCAATTTT
    GGTCATACAA GGTACTCTTT CTTTCGATCT ATTAGAACCC AGAATCAGAC
    TTCATCTCAG AACTCTGGAT CTTTGATAAA ACTCTGAAAT TTGGGATTTT
    TTTTTTACAN ANAAGCGAAG CAGCGTCGCG GTTCAAGACG AGGTCCAGAG
    AAACCGAAGA AGAAGAAGAT TTGACGATTA AACCGGNGGT TCGTATTTCC
    GACGGGAATC TAATCATCAA AAACCGGACG ATTCTAACCG GTNTACCANA
    TAATGTCATC ACGACGTCAG CATCGGAAGC TGGACCGGTA NAAGGAGTCT
    TCGTCGGAGC TGTNTTTAAC AAGGAAGAGA GTAAACNCAT CGTACCGATC
    GGTACGCTTC GCAATTCCCC GGTTTATGTC TTGTTTCCGG GTTAAGCTCT
    GGNGGATGGN CCANAAATGG GANAAATGGG CCGANAATTT NCCT

AT5G20250.1

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.2

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.3

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.4

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1).
Link
InterPro Scan - TAIR