F00643

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F00643

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F00643

Determination of introduced cDNA(s)

RAFL02-06-G14 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL02-06-G14 - Resource: pda02237

full reading [+] show sequence

AAAACAATTC CCACACTATC TTTCCTCCAC ATTAAAGTAA AGTATCTCTC 
TCTTTTTTCT CCTACGTACT CCATAGTGAT GGCTTCATCG CTTCTGACAT 
CTTCCGTTAT GATCCCTACC ACCGGTTCCA CCGTGGTTGG CCGGTCAGTG 
TTACCCTTTC AATCTTCCCT GCACTCTCTC CGCCTGACCC ATTCGTACAA 
GAACCCCGCA TTGTTCATCT CATGTTGCTC TTCTGTGTCC AAAAATGCGG 
CAACTAGTTC TACTGATCTC AAACCCGTTG TGGAACGGTG GCCGGAGTAC 
ATACCGAACA AGCTTCCCGA CGGAAACTAT GTGCGTGTAT TCGACACGAC 
GCTCCGTGAC GGTGAACAAT CTCCTGGTGG ATCCCTCACT CCGCCGCAGA 
AGCTAGAGAT TGCCCGACAG CTCGCTAAAC TCCGAGTAGA CATCATGGAA 
GTCGGTTTTC CGGGATCATC TGAAGAAGAG TTAGAAACCA TTAAGACCAT 
CGCCAAGACT GTGGGGAATG AGGTGGATGA GGAAACAGGT TACGTCCCTG 
TGATATGCGC CATAGCTCGA TGCAAACATA GAGACATTGA GGCGACTTGG 
GAGGCGCTGA AGTACGCGAA GAGGCCAAGG ATACTCGTAT TCACATCTAC 
TAGTGACATT CACATGAAAT ATAAGTTGAA AAAGACTCAA GAAGAAGTGA 
TTGAGATGGC CGTGAGTAGT ATTAGGTTTG CTAAAAGCTT GGGCTTCAAT 
GACATCCAGT TTGGGTGCGA AGATGGCGGC AGGTCGGACA AGGATTTCCT 
ATGCAAGATT CTAGGAGAAG CCATAAAAGC CGGTGTTACG GTGGTGACCA 
TCGGTGATAC GGTAGGGATC AACATGCCAC ATGAATACGG GGAACTCGTG 
ACTTATCTCA AAGCAAACAC CCCTGGAATT GACGATGTTG TCGTCGCTGT 
TCATTGTCAC AACGACCTTG GTCTTGCAAC CGCCAACTCA ATCGCCGGTA 
TACGTGCGGG AGCAAGACAG GTCGAAGTAA CTATCAACGG AATTGGTGAA 
AGAAGTGGCA ATGCGTCGCT TGAGGAGGTC GTGATGGCTT TGAAATGTCG 
AGGGGCATAT GTGATCAATG GGGTTTACAC AAAAATAGAC ACACGCCAAA 
TCATGGCTAC CAGCAAGATG GTTCAAGAGT ACACGGGCTT GTATGTTCAA 
GCACATAAGC CCATAGTTGG AGCGAACTGT TTTGTTCATG AGAGCGGCAT 
TCATCAGGAT GGAATATTGA AAAATAGGAG TACTTATGAG ATCTTATCAC 
CAGAAGATAT TGGGATTGTA AAATCTCAAA ATTCCGGCCT TGTTCTTGGA 
AAGCTTAGTG GACGTCACGC TGTGAAAGAT CGGCTGAAAG AGTTGGGATA 
TGAACTCGAT GATGAGAAAT TGAACGCTGT CTTCTCACTA TTCAGAGATT 
TAACCAAGAA TAAAAAGAGA ATCACGGATG CTGATTTGAA GGCATTAGTA 
ACATCTAGCG ATGAAATCTC TTTGGAGAAA TTAAACGGCG CTAACGGTTT 
AAAGTCTAAC GGCTATATAC CAGTTCCTCA GGTTTCATCG AATGTGTAAA 
CACGTTGTAT CTCTTGTATA TTTGTGTACC ACAGTAATAT CAATTTCCAT 
TTGATAATGC CGTTTTTGTT GTAATGTATT AAAAACAGTA TTAAATAAAA 
GGAACTGCTT TATTTCCT

Gene models with high sequence identity

AT5G23010.1 [+] show detail - methylthioalkylmalate synthase 1
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
ProfileScan	Pyruvate carboxyltransferase	42.756
Gene3D	Aldolase-type TIM barrel	6.2e-74
superfamily	Aldolase	1.3e-93
HMMPanther	ISOPROPYLMALATE SYNTHASE	2.6e-167
HMMPanther	ISOPROPYLMALATE SYNTHASE RELATED	2.6e-167
PatternScan	Alpha-isopropylmalate/homocitrate synthase, conserved site	0
PatternScan	Alpha-isopropylmalate/homocitrate synthase, conserved site	0
HMMPfam	Pyruvate carboxyltransferase	5.2e-74

See the detailed result >

AT5G23010.1

Model type: Protein coding
Short Description: methylthioalkylmalate synthase 1
Curator Summary: Encodes a methylthioalkylmalate synthase, catalyzes the condensation reactions of the first two rounds of methionine chain elongation in the biosynthesis of methionine-derived glucosinolates.
Computational Description: methylthioalkylmalate synthase 1 (MAM1); FUNCTIONS IN: 2-(2'-methylthio)ethylmalate synthase activity; INVOLVED IN: glucosinolate biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), Alpha-isopropylmalate/homocitrate synthase, conserved site (InterPro:IPR002034), Pyruvate carboxyltransferase (InterPro:IPR000891); BEST Arabidopsis thaliana protein match is: 2-isopropylmalate synthase 2 (TAIR:AT5G23020.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR