F14612

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F14612

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Original: F14612

Determination of introduced cDNA(s)

  • RAFL09-96-G12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-96-G12 - Resource: pda17927

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-96-G12 - full reading 
    GGTAAAAAAA CTCACCAAAC GAGGAAAGCC AAAGAGAGAG AGAGAGAAAC 
    AAAAATGGCG TCACAGAGTT GCTACAATGG AATCCATCGT ATAAAAAGGG 
    TTCATCCTCT GATTCACTCT CTCACATTTC ATCGATTCTC CTCATCTTCT 
    CTTCCATTCA AATCTCACAT TCCCTCTTCA CAACAACAGC TTCGTATTCG 
    CTCATCTTCT TCGCTTTCTG ATCTTGGAAA TCGCAGGCAT TGTTCGTCTA 
    CTCGAGGATT TCAATTTTGG TCATACAAGA GAAGCGAAGC AGCGTCGCCG 
    TTCAAGACGA GGTACAGAGA AAACGAAGAA GAAGAAGATA TGACGATTAA 
    ACCGGCGGTT CGTATTTCCG ACGGGAATCT AATCATCAAA AACCGGACGA 
    TTCTAACCGG TGTACCAGAT AATGTCATCA CGACGTCAGC ATCGGAAGCT 
    GGACCGGTAG AAGGAGTCTT CGTCGGAGCT GTATTTAACA AGGAAGAGAG 
    TAAACACATC GTACCGATCG GTACGCTTCG CAATTCCCGG TTTATGTCTT 
    GTTTCCGGTT TAAGCTCTGG TGGATGGCCC AGAGAATGGG AGAAATGGGC 
    CGAGATATTC CCTACGAGAC GCAGTTCTTA TTGGTCGAGA GCAACGATGG 
    GTCCCACCTT GAGTCTGACG GAGCTAACGG CGTCGAGTGT AACCAGAAAG 
    TTTACACCGT TTTCTTGCCG TTAATCGAAG GATCTTTCCG TTCGTGTCTC 
    CAAGGAAACG TTAACGATGA GGTTGAGCTC TGTTTGGAGA GTGGTGACGT 
    GGACACTAAA CGGTCGTCGT TTACTCACTC TCTGTATATT CACGCCGGTA 
    CAGATCCGTT TCAGACAATA ACGGACGCTA TTCGCACCGT TAAGTTGCAT 
    CTGAATAGTT TCCGTCAACG TCACGAAAAG AAGCTTCCAG GGATCGTTGA 
    CTACTTCGGA TGGTGCACTT GGGACGCGTT TTATCAAGAA GTGACTCAGG 
    AAGGCGTCGA AGCTGGTCTT AAGTCTCTCG CCGCCGGTGG TACGCCGCCG 
    AAGTTTGTTA TCATAGACGA CGGTTGGCAA TCAGTTGAGA GAGATGCCAC 
    GGTGGAGGCC GGAGATGAGA AGAAAGAGTC ACCGATTTTT CGACTGACGG 
    GGATCAAGGA GAACGAAAAG TTTAAGAAGA AGGATGATCC AAACGTTGGG 
    ATTAAGAACA TTGTCAAGAT TGCTAAGGAG AAACACGGTT TGAAATATGT 
    TTACGTGTGG CACGCGATAA CGGGTTATTG GGGCGGAGTT AGACCCGGGG 
    AAGAATACGG ATCTGTTATG AAATATCCTA ATATGTCAAA GGGTGTGGTG 
    GAGAATGATC CGACGTGGAA GACTGATGTA ATGACGCTGC AAGGGTTGGG 
    TTTGGTTAGC CCCAAGAAAG TGTATAAGTT TTACAATGAG CTTCATAGTT 
    ACTTGGCTGA TGCTGGCGTG GACGGTGTGA AAGTGGATGT GCAGTGTGTA 
    TTGGAGACTT TGGGTGGTGG TTTAGGCGGT CGGGTTGAGC TGACTCGTCA 
    GTTTCATCAA GCTCTTGACT CCTCTGTTGC TAAGAACTTC CCGGATAATG 
    GCTGCATTGC TTGTATGAGC CATAATACGG ATGCTCTTTA CTGCTCGAAG 
    CAAGCAGCTG TGATTAGAGC ATCAGATGAT TTCTATCCAC GGGATCCGGT 
    GTCTCATACC ATCCATATAG CCTCCGTTGC TTATAACAGT GTGTTCTTGG 
    GAGAGTTTAT GCAGCCTGAC TGGGATATGT TCCATTCCGT GCACCCTGCT 
    GCAGAGTATC ATGCCTCTGC TAGGGCCATC AGTGGTGGGC CTCTCTATGT 
    CAGTGATTCT CCTGGAAAGC ACAACTTTGA GCTTCTAAGA AAGCTTGTAT 
    TGCCTGATGG ATCGATTCTT CGTGCTCGAC TCCCTGGTAG ACCAACTCGC 
    GATTGTTTGT TCGCTGATCC TGCCCGTGAT GGTGTCAGCT TGCTAAAGAT 
    ATGGAACATG AACAAGTACA CTGGAGTTCT TGGCGTGTAT AACTGCCAAG 
    GAGCAGCTTG GAGCAGCACA GAGAGAAAAA ACATTTTCCA CCAGACTAAA 
    ACTGATAGCC TCACTGGCTC CATTCGTGGT CGTGATGTGC ATTCAATATC 
    GGAGGCCTCC ACTGATCCAA CAACCTGGAA TGGAGACTGT GCTGTTTACT 
    CCCAGAGCAG AGGCGAACTT ATTGTTATGC CATACAATGT GTCTCTTCCA 
    GTCTCACTCA AAATCCGTGA GCACGAGATC TTCACGGTGA GCCCCATTAG 
    TCATCTTGTT GATGGTGTAT CTTTTGCCCC AATTGGTCTA GTAAACATGT 
    ACAATTCGGG AGGAGCTATC GAAGGACTTA GATATGAAGC CGAGAAGATG 
    AAAGTGGTAA TGGAAGTTAA AGGATGTGGC AAATTCGGAT CTTACTCTTC 
    TGTGAAGCCT AAGAGATGCG TTGTTGAGTC AAATGAGATT GCATTCGAGT 
    ACGATTCCTC CTCTGGATTG GTCACCTTTG AGTTAGACAA AATGCCTATA 
    GAGAACAAAC GATTTCATCT GATCCAAGTT GAGTTATGAA GAGTGATTCA 
    TACAATTCAT TAAACAAAAA CAGTCTCAAA ACAGTTTTAA ATAGTCTTTA 
    TTATTTGTAA ACCTTTGGTT ACCATTGTAG CTACTTTCAA TGTTATACTA 
    CAGTTCACGT TAAAAAAAAG A

    Gene models with high sequence identity

    1. AT5G20250.4 [+] show detail - Raffinose synthase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Raffinose synthase 0
    superfamily Glycoside hydrolase, catalytic core 9.6e-60

    See the detailed result >

    AT5G20250.4

    Model type
    Protein coding
    Short Description
    Raffinose synthase family protein
    Curator Summary
    encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
    Computational Description
    DARK INDUCIBLE 10 (DIN10); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1).
    Link
    InterPro Scan - TAIR