F27131

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27131

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27131

Determination of introduced cDNA(s)

  • RAFL09-07-E24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-07-E24 - Resource: pda07952

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-07-E24 - full reading 
    AGCTACTACT GTTGAAGCGA TTCTCACTAA AACCCTCGAA CACATCGCCT 
    TTATCTCTTT CTCTAGATCT ACTCGCTATG GCTACTATCA CCGTTGTTAA 
    GGCTAGACAG ATCTTCGACA GTCGTGGTAA TCCCACCGTT GAGGTTGATA 
    TCCACACGTC AAATGGTATT AAGGTTACAG CAGCTGTTCC AAGTGGAGCT 
    TCCACTGGTA TCTATGAGGC TCTTGAGCTG AGGGATGGAG GATCTGACTA 
    CCTTGGAAAG GGTGTATCTA AGGCTGTTGG CAATGTGAAC AACATCATCG 
    GGCCAGCACT TATTGGAAAG GACCCAACTC AGCAGACTGC TATTGACAAC 
    TTCATGGTCC ATGAACTTGA CGGAACCCAA AACGAGTGGG GGTGGTGCAA 
    GCAAAAGCTT GGAGCCAATG CGATTCTTGC TGTGTCTCTT GCTGTCTGCA 
    AAGCTGGGGC TGTTGTCAGC GGCATTCCTC TATACAAGCA CATTGCCAAC 
    CTTGCTGGTA ACCCCAAGAT TGTGCTACCA GTTCCTGCCT TCAACGTCAT 
    CAATGATGGA TCCCATGCCG GAAACAAGCT TGCTATGCAG GAGTTTATGA 
    TCCTCCCTGT TGGAGCTGCT TCTTTCAAGG AAGCCATGAA GATGGGTGTG 
    GAAGTTTACC ACCACTTGAA GTCTGTGATT AAGAAGAAGT ACGGCCAGGA 
    TGCCACAAAT GTTGGTGATG AAGGTGGGTT TGCACCAAAC ATTCAGGAGA 
    ACAAGGAGGG TCTTGAATTG CTCAAGACTG CTATCGAGAA GGCTGGATAC 
    ACTGGAAAGG TTGTCATTGG AATGGATGTT GCCGCTTCAG AGTTCTACTC 
    AGAAGACAAG ACCTACGACT TGAACTTCAA AGAAGAGAAC AACAATGGCT 
    CTCAGAAGAT TTCTGGTGAT GCTCTAAAGG ACCTGTACAA GTCCTTTGTC 
    GCTGAGTACC CAATCGTGTC CATTGAGGAC CCATTTGACC AAGATGACTG 
    GGAGCACTAT GCTAAGATGA CCACTGAGTG TGGAACCGAG GTTCAGATTG 
    TCGGTGATGA TTTGTTGGTC ACTAACCCCA AGAGAGTTGC TAAGGCAATC 
    GCAGAGAAGT CTTGCAATGC TCTTCTTTTG AAGGTTAACC AAATCGGATC 
    TGTAACCGAG AGTATCGAGG CAGTTAAGAT GTCGAAGAAA GCAGGTTGGG 
    GAGTGATGAC CAGCCACAGA AGTGGTGAAA CCGAGGACAC ATTCATTGCT 
    GACTTAGCCG TTGGCTTGTC CACTGGACAA ATCAAAACCG GTGCTCCTTG 
    CAGATCCGAG CGTCTTGCCA AGTACAACCA GCTTTTGCGT ATTGAGGAGG 
    AGTTGGGATC AGAGGCAATT TACGCTGGAG TCAACTTCCG CAAACCTGTG 
    GAACCCTACT AAATGGAGCT TTTAGAAGCA AAGTGGTCTT CTTTGTGACG 
    AGGAGAAGAT GACCTGAGTT TGATCATTTG CTTTAATTAA ATAAAACGTT 
    CTGTTTTTGT TTCTTCTTTG TTTGGTTTCT TACGTCTTTT GTTGAACCCT 
    TTTTGGGAAA AGTTACTCAT TTTTGTAAGG GAAACATGAG AATGCTCTGC 
    CTTTGTCGAG GACGGTAGCC CCTTATTTCA ATCTAATTTC GGCTTTACTC 
    TCT

    Gene models with high sequence identity

    1. AT2G36530.1 [+] show detail - Enolase
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Enolase 2.6e-236
    PatternScan Enolase, conserved site 0
    HAMAP Enolase 41.855
    HMMPIR Enolase 1.7e-272
    superfamily Enolase C-terminal domain-like 2.8e-128
    superfamily Enolase N-terminal domain-like 1.1e-50
    HMMPfam Enolase, C-terminal 5.3e-160
    HMMPfam Enolase, N-terminal 3.6e-53
    Gene3D no description 3.4e-42
    Gene3D no description 3.5e-139
    FPrintScan Enolase 8.4e-53
    FPrintScan Enolase 8.4e-53
    FPrintScan Enolase 8.4e-53
    FPrintScan Enolase 8.4e-53
    FPrintScan Enolase 8.4e-53
    FPrintScan Enolase 8.4e-53
    HMMPanther ENOLASE 1.7e-124

    See the detailed result >

    AT2G36530.1

    Model type
    Protein coding
    Short Description
    Enolase
    Curator Summary
    Involved in light-dependent cold tolerance and encodes an enolase. Protein is tyrosine-phosphorylated and its phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds.
    Computational Description
    LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2 (LOS2); FUNCTIONS IN: phosphopyruvate hydratase activity, copper ion binding; INVOLVED IN: response to cadmium ion, response to salt stress, response to cold, response to light stimulus, response to abscisic acid stimulus; LOCATED IN: in 8 components; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Enolase (InterPro:IPR000941), Enolase, C-terminal (InterPro:IPR020810), Enolase, conserved site (InterPro:IPR020809), Enolase, N-terminal (InterPro:IPR020811); BEST Arabidopsis thaliana protein match is: enolase 1 (TAIR:AT1G74030.1); Has 13396 Blast hits to 13370 proteins in 3710 species: Archae - 270; Bacteria - 5735; Metazoa - 2292; Fungi - 281; Plants - 265; Viruses - 0; Other Eukaryotes - 4553 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR