K11341

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K11341

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K11341

Determination of introduced cDNA(s)

  • J013032A17 - Rice Full-length cDNAs - Original: J013032A17

    full reading [+] show sequence

    Nucleotide sequence: J013032A17 - full reading 
    GTGCAGCACA CTAATCGATC ACCGCACTAT ACACAATACG ACCATTAGCT 
    ATTACTAGCT AGCTATATAT CCCTCCCTTG TTTCTTTCGA GCAAGAGCGA 
    TGGAAACGGC GCCGGCCGCG AACGGTTGTA GTGGCGGCCG CCGGCTGCAG 
    GAAGAAGAGC CCGCAGCTCG GTGGTGCGCG GTGACCGGTG GCCGCGGCTT 
    CATGGCGAGG CACCTCGTGG CGGCGCTGCT CCGCTCCGGC GAGTGGCGCG 
    TGCGGATCAC CGACCTCGCG CCGGCGATCA CCCTTGACCC CGTCGAGGAG 
    AAGGGGCTCC TCGGCGAGGC CCTCCGCGAC GGCCACGCCG TCTATGACTC 
    CGTCAATGTT TGCGACATAA CGCAGTTGAC CAAAGCTTTT CAAGGGGTGG 
    AAGTTGTTTT TCACACAGCT GCTGCAGACC CTGACATCAA CGACTTCCAA 
    CTACATTACA AGGTCAATGT TGAAGGGACA AAGAATGTTA TTGATGCTTG 
    TGTAACGTGC AAGGTTAAAA GGCTGATATA CACCAGTTCC AGTGGTGTTG 
    TATTTGACGG AGTTCATGGC CTTTTTGATG TAGATGAATC AATGCCATAC 
    CCAAACAAGT TTCCTGATGC ATATGCACAA AGCAAGGCAG AAGCAGAGAA 
    GTTAGTGAGA AATTCCAACG GCATATGTGA ACTTCTAACT TGTTCCATAC 
    GTCCTGGTAG CATTTTTGGC CCTGGTGACA CCATTGTACC ACATTTACTT 
    TCCCACTGGA GAACGATGTT TATTATCGGC GATGGAATGA ATTGTGATGA 
    TTTTGTATAT GTTGAAAATG TGGTACATGG CCACATTTGT GCTGAGAAAA 
    CTCTCTCGAC TAAAGAGGGT TCAAAGATAA GTGGAGGCAA GAATGTGTGA 
    ATTCATTTTG CTTCGACCAT ATCTAAGACC TCATACATGG AGCAAGTTCT 
    ATTGCTATTG AAGATGTAAA ATTTATTTGA CATGGATGAT AACCAATGGT 
    CGATGGCAAT GATGTCTACA CATATAAGTT TGATGTATTT TTTTTACAAA 
    TGGCTAAGTA TTGTAATCTT GTTGGGAACA ATATTGATTC TTATTGGAGA 
    ATGGGCTTCC ACTTGTTGCA GTCAACATAA TTGTAATATA TATCTGTGAG 
    ATTTTTATTG CTATATCTCT TTATGCAAAA ACATTAAGTC T

    Gene models with high sequence identity

    1. AT1G47290.2 [+] show detail - 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
      E-value: 4e-74; Score: 275.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 3 BETA-HYDROXYSTEROID DEHYDROGENASE 1e-74
    HMMPanther NAD DEPENDENT EPIMERASE/DEHYDRATASE 1e-74
    superfamily NAD(P)-binding domain 4e-45
    Gene3D NAD(P)-binding domain 8.9e-33
    HMMPfam 3-beta hydroxysteroid dehydrogenase/isomerase 5.6e-59

    See the detailed result >

    AT1G47290.2

    Model type
    Protein coding
    Short Description
    3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1
    Curator Summary
    Encodes an enzyme with 3β-hydroxysteroid dehydrogenase/C4-decarboxylase activity in vitro. The activity of the enzyme was determined using microsomal extracts of yeast overexpressing the Arabidopsis gene. Cytosolic fractions failed to be associated to the activity, leading to the speculation that the enzyme is membrane-bound.
    Computational Description
    3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 (3BETAHSD/D1); FUNCTIONS IN: 3-beta-hydroxy-delta5-steroid dehydrogenase activity, sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity; INVOLVED IN: steroid biosynthetic process, metabolic process; LOCATED IN: membrane; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: NAD(P)-binding domain (InterPro:IPR016040), 3-beta hydroxysteroid dehydrogenase/isomerase (InterPro:IPR002225); BEST Arabidopsis thaliana protein match is: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 (TAIR:AT2G26260.1); Has 24445 Blast hits to 24432 proteins in 2873 species: Archae - 619; Bacteria - 15430; Metazoa - 691; Fungi - 529; Plants - 1560; Viruses - 132; Other Eukaryotes - 5484 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR