K12840

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K12840

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K12840

Determination of introduced cDNA(s)

  • J033148L17 - Rice Full-length cDNAs - Original: J033148L17

    full reading [+] show sequence

    Nucleotide sequence: J033148L17 - full reading 
    GGGTAGAAGC AGAGGTAAGC AGGGCAGAGA GTAGAAACAG GAGCACTGCA 
    TTACTGGTAG TGGGAGCGTA AAGCTCTCTC CTCTCTTCTT CTTCTTCTTC 
    TTGGCGTTTT CCCTCATCTC CTTCTACTAG TACTAGTACA TCACCCTCCC 
    ACTCTTCTTC ATCTTCCGCC CAGCGAGGTT CGTCTGGATG TGAGCTGAAT 
    CTCTCCGTCT GCCCAAGTTG ATTGAGTTGG TCGACTGAAT GGGATCAAGG 
    AGACCAAGAC TGCCTGGGTT TGGTGAGGAC TGTGAGCCTC GTGGTGGTGG 
    GAGAGGAGGA GGTGGCCGTG GCCGTGGCAG CTACTACCCA CAGGCACAGC 
    AATACCACCC ACAAGGACAT GGTGGCCGTG GAGGAGCAGG CTACTACCAT 
    GGTGCTGCTC CTCAACCTCG CGGCGCAATG GTGGTGCAGC AATGGCGTCC 
    TGCTACTGCT GCTGCTGAGC ATTTGGGCCA TCAACAGCCC TACAACAGCA 
    GTGTGAGACC ACAGCACTAT TATGGTCCGT CTGCCATAGC TCCCGAGCTG 
    CTCCAAGCAA TGGATGCTCC ACATGAGCCT CCTGCCAATG TCTCCTCACC 
    AGAAGCAGCC TCTCCGGAGG CATCATCACC ACGGTCTCTT GCTCTTGAGG 
    TCACAGAGCA GCTTCAGGAC TTGTCTGTGC AGTACCAATT AAGTGAGAGC 
    CAGGAAGAGA TTGTCCAACA TGTTCCTGTG TCCACCAAAT CATTTAAATT 
    TCCTCACCGC CCTGGAAGTG GGAGTATTGG AACCAGATGT TTAGTGAAGG 
    CGAATCACTT CTTTGCTCAA CTGCCAGACA AGGATCTTCA TCAGTATGAC 
    GTTTCAATCA CCCCGGAGCT TACATCACGA ATTCGGAGCC GTGCTGTGAT 
    GGAAGAGTTG GTGAGACTGC ACAAGATGTC ATACTTGGGA GGACGTCTTC 
    CAGCCTATGA TGGTAGGAAG AGCCTTTACA CGGCCGGTCC ATTGCCGTTT 
    ACTTCAAAAG AATTTCGCAT CTCTTTGCTT GAGGAAGATG ATGGTTCTGG 
    TTCGGAGAGG CGTCAGAAAA CATATAACGT GGTGATTAAG TTTGCTGCAA 
    GAGCTGATCT TCACCGTCTT GAGCAATTTC TAGCTGGAAG GCAGGCAGAG 
    GCTCCCCAGG AGGCCTTGCA AGTTCTTGAT ATTGTCTTGC GAGAGTTGCC 
    AACAGCAAGA TATGCACCCT TTGGTCGATC CTTCTTCTCT CCTGACCTGG 
    GGAGGAGACG ATCCCTCGGT GAGGGACTAG AAACCTGGCG TGGGTTTTAT 
    CAGAGCATTC GTCCTACTCA AATGGGCTTG TCACTGAATA TTGATATGTC 
    GGCAACTGCT TTCTTTGAGC CGTTACCAGT CATAGATTTT GTCATACAGC 
    TTTTAAATAC TGACATCCGC TCGAGGCCCT TATCAGATGC TGAGCGTGTC 
    AAGATCAAGA AGGCCTTAAG AGGAGTGAAG GTAGGAGTTA CTCACCGTGG 
    CAACATGCGC CGGAAGTATC GGATATCTGG TTTGACATCT CAGGCAACTC 
    GGGAACTGAC TTTTCCTGTT GATCAAGGAG GCACAGTGAA ATCTGTTGTA 
    CAATATTTTC AAGAGACATA TGGATTTGCG ATCCAGCATA CCTATCTTCC 
    TTGTCTGCAA GTTGGCAATC AGCAGCGTCC AAATTACCTA CCAATGGAGG 
    TCTGCAAAAT AGTGGAAGGA CAGAGGTACT CCAAGAGACT GAACCAGAAT 
    CAGATAAGGG CTCTTTTAGA GGAGACATGT CAGCGCCCAC ACGATAGGGA 
    GCGTGACATA ATTCAGATGG TGAATCACAA CTCCTACCAT GAAGATCCTT 
    ATGCAAAGGA GTTTGGCATT AAGATCAGCG AGCGTCTGGC CTTGGTTGAG 
    GCACGGATTT TACCTGCCCC TCGGCTCAAG TATAATGAGA CTGGCAGAGA 
    GAAGGATTGC TTGCCTAGAG TTGGTCAGTG GAATATGATG AACAAGAAAA 
    TGGTAAATGG TGGTAGAGTC AGGAGCTGGA TATGTGTCAA TTTTGCTCGA 
    AATGTGCAAG AGAGTGTTGC TAGTGGATTT TGTCGTGAAC TGGCCCGCAT 
    GTGCCAGGCC TCAGGAATGG ACTTTGCTTT GGAGCCTGTT CTTCCATCTA 
    TGTATGCACG TCCTGATCAA GTGGAACGAG CTCTGAAAGC CAGGTTCCAT 
    GATGCAATGA ACATACTTGG GCCACAGCAC AAGGAGCTCG ATTTACTTAT 
    CGGACTGCTT CCTGATAACA ATGGTTCTCT TTATGGTGAT TTGAAGCGTA 
    TATGCGAAAT TGACCTTGGA TTGGTTTCCC AGTGCTGTTG CACAAAGCAA 
    GTGTTTAAAA TGAACAAACA AATCCTAGCA AATCTTGCTC TGAAGATAAA 
    TGCGAAGGTT GGGGGAAGGA ACACTGTACT GGTTGATGCA GTGTCGAGAC 
    GCATTCCGTT GGTAACTGAC AGGCCTACTA TTATATTCGG TGCTGATGTC 
    ACCCATCCTC ACCCTGGCGA AGATAGCAGC CCATCCATTG CTGCTGTTGT 
    GGCCTCCCAA GATTGGCCTG AAGTGACAAA GTATGCTGGT TTAGTTTCTG 
    CTCAATCTCA CAGACAAGAG TTAATAGATG ATCTGTATAA CATCACGCAT 
    GATCCTCATA GAGGGCCCAT CTGCGGTGGA ATGGTCAGGG AACTTCTTAT 
    ATCCTTCAAA AGATCAACTG GTCAAAAGCC TCAACGGATA ATATTCTATA 
    GGGATGGTGT TAGTGAAGGG CAATTTTACC AGGTTCTATT GCATGAGCTT 
    GATGCAATCC GAAAGGCTTG TGCATCACTC GAAGCAAATT ACCAACCGCA 
    GGTGACTTTC ATTGTGGTTC AGAAGCGCCA CCACACGAGA TTATTTGCAC 
    ACAACCACAA TGATCAGAAT TCAGTCGACA GGAGTGGGAA CATATTGCCT 
    GGTACTGTTG TTGACTCCAA GATTTGCCAT CCTACGGAGT TTGACTTCTT 
    CTTGTGCAGC CATGCTGGAA TCAAGGGCAC AAGCCATCCC GCTCATTACC 
    ACGTCCTGTG GGATGAAAAC AACTTCACAG CTGATGCGTT GCAGACCCTC 
    ACCAACAACC TCTGTTACAC TTATGCGAGG TGCACACGAT CCGTATCTAT 
    TGTTCCACCA GCATACTATG CTCATCTGGC GGCGTTCAGA GCCCGTTTCT 
    ACATGGAGTC AGATAGCTCG GACAGTGGTT CAATGGCGAG TGGTCGTGGA 
    GGAGGTTCGT CTACATCGCG CAGCACTCGT GCTGCAGGTG GTGGAGCCGT 
    CAGGCCCCTT CCTGCACTCA AGGACAGCGT CAAGAATGTC ATGTTCTACT 
    GTTAGAATTC TACTACTGCT TCGCTTGGCT ACTACTATCA GTTAGCCTTT 
    AAATTGCCTC CCATTTGATT GCACTTAGTT GTTCATCATG GGAAGCCTCA 
    AATAAGAAGT ATGCAAGACT TCTGAAGCCA CATGCTATCA TGTTGTGTGT 
    TTCTGGGACA CACCATTCTA TTTCACATTA TCTGTAGACT CTATCCCACT 
    ATTTCATAAA AGCTGGGCTT CCCCTGGCTC TATTTGTACT CTAATTCGTA 
    TGGACCTGTT TCATCATTTC AAAATGGTCA ATTGTCTTGT TTGC

    Gene models with high sequence identity

    1. AT1G48410.1 [+] show detail - Stabilizer of iron transporter SufD / Polynucleotidyl transferase
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    superfamily PAZ domain 2.5e-58
    superfamily Polynucleotidyl transferase, ribonuclease H fold 2.9e-24
    ProfileScan Argonaute/Dicer protein, PAZ 31.73
    ProfileScan Stem cell self-renewal protein Piwi 57.229
    Gene3D no description 1.1e-44
    Coil coiled-coil 0
    HMMPanther EUKARYOTIC TRANSLATION INITIATION FACTOR 2C 3.1e-289
    HMMPfam Stem cell self-renewal protein Piwi 1.8e-108
    HMMPfam Argonaute/Dicer protein, PAZ 1.6e-34
    HMMPfam Domain of unknown function DUF1785 2.3e-22

    See the detailed result >

    AT1G48410.1

    Model type
    Protein coding
    Short Description
    Stabilizer of iron transporter SufD / Polynucleotidyl transferase
    Curator Summary
    Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.
    Computational Description
    ARGONAUTE 1 (AGO1); FUNCTIONS IN: protein binding, endoribonuclease activity, siRNA binding, miRNA binding; INVOLVED IN: in 11 processes; LOCATED IN: cytosol, nucleus, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Domain of unknown function DUF1785 (InterPro:IPR014811), Stem cell self-renewal protein Piwi (InterPro:IPR003165), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337); BEST Arabidopsis thaliana protein match is: Stabilizer of iron transporter SufD / Polynucleotidyl transferase (TAIR:AT5G43810.2); Has 29249 Blast hits to 15917 proteins in 1190 species: Archae - 22; Bacteria - 5845; Metazoa - 12576; Fungi - 2412; Plants - 4639; Viruses - 404; Other Eukaryotes - 3351 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR