K24042

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K24042

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K24042

Determination of introduced cDNA(s)

  • J023099J24 - Rice Full-length cDNAs - Original: J023099J24

    full reading [+] show sequence

    Nucleotide sequence: J023099J24 - full reading 
    GACTCGCCTC CGTCGCGGTG AGCGAGCGAG CGCGAGCGGC GATGGAGGAG 
    ACGAGGAGGA GGGGTGGTCG AATGTGAGAG GCGAATCCAC GTCTTGCCGC 
    TGAGAAGGCG GCGGGAGGGA GCAACGTCGT GAGACCCACC ACGCGGTCGC 
    TGACGGGTGG GCCCAACCAC CCGGCCGCCG CCACTCCCGC GCCCTTATCA 
    TCTATCTGCC TCGTCTTCTC CGCCCCCTCA AGCTGTTCGA CGGAATGGCG 
    CAGTGGGACG CGCTCGTGGA CGCCGCGCTG GCGAGGCTGG CGTCCAGGAG 
    CCTCCTGCGC GCCACGCGCC CCATCGCCCT CGCGCCGCCG CCCGCGCCGC 
    CGGAGACCTT CCCCTGCCCA GGCCCGTGGG ACCGCGCAGC CGTCGAGATC 
    CGGCTCGACC GCGCAACGCT CCACCAATGG CTGGCTGAAG GTGGTGAGGG 
    GAGTGGCCAA GAAGAGAAAG TGGACGAGAA ACTGATTCTT TTCTCCGGGA 
    ATGATTACAT GGGCCTCAGC TCGCATCCTG CAATTCGCCA TGCCGCAGTG 
    AAGGCAGCTG AAGAGTACGG CATGGGCCCT AGAGGATCTG CGTTGATTTG 
    TGGATATACT ACATATCACA AAATGGTGGA GGAATCGTTG GCAGAGCTGA 
    AGAAAAAGGA GGATTGCCTT CTTTGTCCCA CTGGATTTTC TGCCAATATG 
    GCTGTAATGA CGGCTCTGGG AAACATCAGC TCTCTGTTGG CTGCTGGGAG 
    AAAACCTGCG GAGGACGAAA GAATAGCAAT TTTCTCAGAT GCTCTGAACC 
    ATGCTTCGAT CATTGATGGG ATTCGCCTTG TTGAGCGACA GCAAGAAGTG 
    GTATCATTTG TGTACAAGCA CTGTGACATG TCCCATCTTG AACTTTTGCT 
    ATCCAGTTGC TCAATGGAAA AGAAAGTTGT TGTCACAGAT AGCCTGTTCA 
    GCATGGATGG TGATTTTGCT CCATTGCCTG AGCTTGTCAA GTTGCGCAGA 
    AAGTATGGGT TTTTGTTGGT GATCGACGAT GCACATGGAA CACTTGTTTG 
    TGGTGAGAAT GGTGGTGGTG CACCTGAGCT GTTTGAATGT GAGAATGAGA 
    TTGACATAAG TGTTGGCACC TTAAGCAAGG CTGCTGGCTG CCAGGGTGGT 
    TTTATAGCAT GCAGTACCCG ATGGAAGAGG CTGATTCAAT CGCGAGGTCG 
    TTCCTTCATA TTTTCAACTG CTTTACCAGT GCCAGTAGTT GCTTCTGTGT 
    ATGCTGCCCT CTATGTATCA AGGGAGGAGA GATGGCGGAG ATCAGTGATC 
    TGGAGGCATG TGCAGTATTT TGCCTCTTTG ACAAAACTCA ACATAACAAG 
    TCCCATAATT TCCATTGTAG TTGGAAGTGA AAAAGCAGCA CTTAGAGCTG 
    GAAGGGATAT GCTAAGATCT GGATTTCATG TCACGCCAAT CCGACCACCC 
    ACAGTACCGC CCAACTCATG CAGGTTGCGA ATAACTCTCA GTGCTTCTCA 
    TTCTTCGGAT GACATCAAAA GGCTGGTGGA TGCGCTGACG CCGTGGCTCC 
    CTCACAAACA TGATATTCAG ATATATGCTG TGGCATCAAA GCTCTAAACA 
    ATGATTCATC CGAGTGTTCA GCTCATCAAG CTTCAAGAAG TAACATTAGC 
    TGAGATCCCA CCGGATGATA TGGGAGAAAA GGGGATAAGT TAGTGCTGGT 
    TGACGCGATT AATACTGGGA TATCTTTGTA TTACAGCACA ATCAATATGA 
    ATATGAAACA TAACTGATGA AACAGTACTA CATCTTTTGC CCTC

    Gene models with high sequence identity

    1. AT5G04620.2 [+] show detail - biotin F
      E-value: 6e-145; Score: 511.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Aminotransferase, class I/II 3.8e-48
    HMMPanther 8-AMINO-7-OXONONANOATE SYNTHASE 3.6e-256
    HMMPanther CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANO 3.6e-256
    superfamily Pyridoxal phosphate-dependent transferase, major domain 2e-74
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 3.2e-69

    See the detailed result >

    AT5G04620.2

    Model type
    Protein coding
    Short Description
    biotin F
    Curator Summary
    The cDNA encoding 7-keto-8-aminopelargonic acid (KAPA) synthase, the first committed enzyme of the biotin synthesis pathway has been cloned and its molecular function confirmed (functional complementation of an E. coli mutant). The subcellular localization of the enzyme (cytosol) proves that the biotin biosynthesis in plants takes place in different compartments which differs from the biosynthetic route found in microorganisms.
    Computational Description
    biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR