K25942

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K25942

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K25942

Determination of introduced cDNA(s)

  • J023125E21 - Rice Full-length cDNAs - Original: J023125E21

    full reading [+] show sequence

    Nucleotide sequence: J023125E21 - full reading 
    GATCGGACTC GGAGCAAAAC ACAATAGAGG AGCAGAGCAG AGCACAGTGA 
    GCTAGTAGCA GAGGCCACAC TAGCGTTGAG ATGGCGCGCG AGGCGGAGAA 
    GGACGCGGCG GCGGCGGCGG AGATCCCGCT GCTGACGCCG TACAAGATGG 
    GGCGGTTCGA GCTCTCCCAC CGTGTGGTGC TCGCGCCGCT CACGCGGAAC 
    CGCTCCTACG GCAACGTGCT GTACTACACG CAGCGCGCCA CCAGCGGCGG 
    CCTCCTCGTC ACGGAGGCCA CCGGCGTCTC CGACACCGCG CAGGGCTACC 
    CGGACACCCC CGGCATCTGG ACGCAGCAGC AGGTCGAGGC GTGGAAGCCC 
    ATCGTCGACG CCGTCCACCG CAAGGGCGCC CTCTTCATCT GCCAGCTCTG 
    GCACGTCGGC AGGGTCTCCA CCAACGAATA CCAACCTGAC GGGCAGGCGC 
    CCATCTCAAG CACAGACAGG CAGATCACAC CGGACGATTC AGGCATCGTC 
    TACTCGAAAC CCCGACGGCT GAGGACGGAG GAGATCCCTC AGATCATCGA 
    CGACTTCAGG CGAGCCGCGC GGAACGCCAT CGAGGCCGGG TTCGACGGCG 
    TGGAGATCCA CGGCGCGCAC GGCTACCTCC TGGAGCAGTT CATGAAGGAC 
    AGCGCCAACG ACCGCAGCGA CGAGTACGGT GGCAGCCTCG AGAACCGGTG 
    CCGCTTCGTT GTCGAGGTGA TCGACGCCAT CGTCGCCGAG GTTGGAGCGC 
    ACCGTGTGGG GATCAGGCTG TCACCGTTCA TCGACTATAT GGACTGCGTC 
    GACTCCGACC CGGTGGCTCT CGGCTCGTAC ATGGTGCAGC AGCTCAACAA 
    GCACCCGGGG TTTCTCTACT GCCACATGGT GGAGCCAAGA ATGGCCATCG 
    TGGAAGGACG CAGGAAGATC ACCCATGGCC TCCTGCCATT CCGGAAGCTG 
    TTCAATGGCA CTTTCATCGC TGCCGGAGGA TACGATCGGG AGGAAGGAAA 
    CAAGGTGATA GCCGATGGAT ATGCTGACCT CGTCGCCTAC GGGAGGCACT 
    TCTTGGCCAA TCCGGACTTG CCCAAGAGGT TCGCGATCAA CGCACCATTG 
    AACAAGTATA ATAGGTCCAC CTTTTACATA CAGGATCCTG TTGTTGGCTA 
    CACGGACTAT CCTTTCCTTG ATGAGAAAGA CGAGGGCGCT GCTACTTATG 
    CTTAATCCTC AATTAAATTG TCAAATTGAA ATGTTCGGTT TAGTAATGTG 
    CACTATATTT GTGATACACT CATAAGTGAC CCTCACTTCC ATCCG

    Gene models with high sequence identity

    1. AT1G76690.1 [+] show detail - 12-oxophytodienoate reductase 2
      E-value: 3e-136; Score: 482.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily FMN-linked oxidoreductases 1e-128
    HMMPfam NADH:flavin oxidoreductase/NADH oxidase, N-terminal 2.2e-88
    HMMPanther 12-OXOPHYTODIENOATE REDUCTASE OPR 4.9e-247
    HMMPanther NADH OXIDOREDUCTASE-RELATED 4.9e-247
    Gene3D Aldolase-type TIM barrel 7.9e-119

    See the detailed result >

    AT1G76690.1

    Model type
    Protein coding
    Short Description
    12-oxophytodienoate reductase 2
    Curator Summary
    Encodes one of the closely related 12-oxophytodienoic acid reductases. This enzyme is not expected to participate in jasmonic acid biosynthesis because during in vitro assays, it shows very little activity with the naturally occurring OPDA isomer. Shows activity towards 2,4,6-trinitrotoluene. Expressed predominately in root. Predicted to be a cytosolic protein.
    Computational Description
    12-oxophytodienoate reductase 2 (OPR2); CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), NADH:flavin oxidoreductase/NADH oxidase, N-terminal (InterPro:IPR001155); BEST Arabidopsis thaliana protein match is: 12-oxophytodienoate reductase 1 (TAIR:AT1G76680.1); Has 13197 Blast hits to 13176 proteins in 2056 species: Archae - 127; Bacteria - 9811; Metazoa - 29; Fungi - 865; Plants - 452; Viruses - 0; Other Eukaryotes - 1913 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR