K26319

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K26319

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K26319

Determination of introduced cDNA(s)

  • J023120O04 - Rice Full-length cDNAs - Original: J023120O04

    full reading [+] show sequence

    Nucleotide sequence: J023120O04 - full reading 
    TTGCAGATGA AGAGAAGCGG ATATTGTATG AGAAGGCAGA GGTGACTGAT 
    TATGAGTTGA TTCCTGGAGG CCGAAACATC AAGGTCACCG AGGAGAACAA 
    GCATGAATAT GTGAACCGGG TTGCAGAACA TCGTTTAACC ACTGCTATTA 
    GGCCTCAAAT CACCTCTTTT ATGGAGGGAT TTAATGAGCT CATTCCTGAG 
    GAGCTGATAT CAATCTTTAA TGACAAAGAA CTTGAACTGC TAATCAGTGG 
    ACTCCCAGAC ATTGACTTGG ACGATCTAAA AGCAAATACA GAATATTCTG 
    GGTACAGCAT AGCTTCTCCA GTCATTCAGT GGTTCTGGGA GATTGTCCAA 
    GGGTTCAGCA AGGAGGACAA AGCCCGGTTC CTTCAGTTTG TTACTGGCAC 
    CTCAAAGGTA CCTCTGGAAG GTTTCAGTGC ACTCCAAGGA ATATCTGGAC 
    CACAACGATT CCAGATACAC AAGGCCTACG GAAGCACCAA CCATCTGCCT 
    TCAGCACATA CTTGCTTTAA CCAACTAGAC CTTCCTGAGT ACACATCGAA 
    AGAGCAGCTC CAGGAGAGAT TGCTACTGGC TATTCATGAG GCGAATGAAG 
    GTTTCGGATT TGGTTAATCA GTCACTCCTG CACCTGTGTG CAAGAAATTT 
    CAGGGAGTAA TGTACAGATA CCGTCGGAGT TGCAATAGGC GAGGGGAATG 
    TGCGCGGACT CTTACATAAC CTGCTACTAG ATTCATTTGT TGCCTGCATC 
    AACCATCGGC GTTGGTCCCT GAAGACTGAT GAGATTTGTT GACAAAGTAC 
    CGGCCTGCCC ACGATGCTTT ATAGGACTGG TTGCTGCGAA GGATGTGCAG 
    GGAGGTGTAG CAGGAAGTGC TAGAAGACAG CAACTATTTG GTGTTCATAA 
    TATTTTTTTT CTTTCCCTTT TGGGTCTTTT TTGGCCATTG CCCCGTTAAT 
    AGATTTCACC TTCTCTATAC ATTGGACCTG TATGGAATTT TTTTCCTTTT 
    TTATTCAAGT TTGTTTTTGG GGGGCATAGA CCGGTGGAAT GCAACATTAA 
    GTAGAATGCA AGTTTTCCAT CGCTATTGCA TATTCAATGC ACATTGACTA 
    AAAGTGTCTG GAGCCACGGG CTGCGGCTAT AAATTTTACT CCT

    Gene models with high sequence identity

    1. AT1G70320.1 [+] show detail - ubiquitin-protein ligase 2
      E-value: 3e-96; Score: 348.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily HECT 3.4e-75
    HMMPfam HECT 4.2e-72
    HMMSmart HECT 5.5e-38
    ProfileScan HECT 66.581
    HMMPanther E3 UBIQUITIN PROTEIN LIGASE URE-B1 6.6e-110
    HMMPanther HECT DOMAIN UBIQUITIN-PROTEIN LIGASE 6.6e-110

    See the detailed result >

    AT1G70320.1

    Model type
    Protein coding
    Short Description
    ubiquitin-protein ligase 2
    Curator Summary
    encodes a ubiquitin-protein ligase-like protein containing a HECT domain. There are six other HECT-domain UPLs in Arabidopsis.
    Computational Description
    ubiquitin-protein ligase 2 (UPL2); FUNCTIONS IN: ubiquitin-protein ligase activity; INVOLVED IN: protein ubiquitination, ubiquitin-dependent protein catabolic process; LOCATED IN: cytosol, mitochondrion, ubiquitin ligase complex, nucleus; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-associated/translation elongation factor EF1B, N-terminal (InterPro:IPR000449), Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote (InterPro:IPR015940), E3 ubiquitin ligase, domain of unknown function DUF913 (InterPro:IPR010314), HECT (InterPro:IPR000569), Armadillo-type fold (InterPro:IPR016024), Ubiquitin interacting motif (InterPro:IPR003903), E3 ubiquitin ligase, domain of unknown function DUF908 (InterPro:IPR010309), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: ubiquitin-protein ligase 1 (TAIR:AT1G55860.2); Has 34660 Blast hits to 19733 proteins in 1213 species: Archae - 286; Bacteria - 8191; Metazoa - 9374; Fungi - 5439; Plants - 1788; Viruses - 520; Other Eukaryotes - 9062 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR