K27512

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27512

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27512

Determination of introduced cDNA(s)

J023036E18 - Rice Full-length cDNAs - Original: J023036E18

full reading [+] show sequence

GGTGCAAATC AAATCACAGC AATGCATGAT TCGCAAAAAA GCCTCCCCAC 
ACTGCCTACA TGACGTCAGC GGTTCTTGCC CTCACCTCTC GATTCTTCTC 
CACTCGAGCA GAAGACCGGA ATCAGCTCCC GTACCCTGTT TGTTACCCAT 
GGAAGCGAAA ATTCCCCTCC TTACTCCTCA CACCATGGGG AGATTCCACC 
TCGCCCACAG GGTTGTCCAT GCGCCGCTGA CCAGATCGAG GTGCTACAAT 
AATCTTCCTC AAGAACATGT TCAGCTGTAC TACTCGCAGA GGGCCACAAA 
TGGCGGCCTT CTGATCGCTG AAGCCACCGG GGTCTCGGAG ACTGCTCAAG 
GGTATCCAAA CACACCGGGT ATATGGACAA AGGAGCAGGT GGAAGCTTGG 
AGGACGGTTG TGGATGCAGT GCATCAGAAG GGTGGTGTTT TCTTCTGCCA 
AATCTGGCAT GTAGGGAGGG CATCTACCAA TGATTATCAG CCCAATGGAC 
AAACACCAAT TCCTTGTACA GACAAGAAAA TCACTCCTAC AGTTCTCAAA 
GATGGAACTG TCGAGGAATT CTCTGCCCCA AGGAGGCTAA GAGAAGATGA 
GATCCCCCAA ATTGTTGATG ATTTCCGAAT TGCTGCTAGG AATTGCATTG 
AAGCAGGCTT TGACGGAGTT GAAATCCACT GCGCATTTGG GTACCTAATT 
GAACAGTTCA TGAAAGATGG TGTCAACGAC AGAACTGACA AGTACGGAGG 
AAGCATCGCA AACCGTTGCC GCTTTGCTCT AGAGGTAATC CAAGCAGCCA 
TCGATGAGAT CGGATCGGAC AGAGTAGGTG TCCGTCTCTC GCCATACTCA 
AACTGCCTGG ACTGTTGGGA CTCAGACCCA GATGCACTTG GCCTCTACAT 
GATCCAAGCC ATGAGCAAGC TGGGCGTTCT CTACTGCAGC ATGGTTGAGC 
CTGAGGTGGT CAAAGTAGAC GGCAAGGTGC AGATTCCTTA CAAGCTATGG 
CACTTCAGGA AAGTGTTTGC TGGCACTTTC ATCGTCACTG GAGGATACAA 
CAGAGAAGAA GGCAATAGAG CAGTGTCTCA GGGTTACACA GACCTGGTAG 
CATATGGTAA ATGGTTCTTG GCAAACCCGG ATCTGCCTAG GCGGTTTGAG 
CTGAATGCGC CTCTGAACAA GTATGACAGA TCGACGTTCT ACACTTCTGA 
TCCAGTTATC GGGTACACCG ATTACCCTTT TCTCAGTCCT CTGTGAGTAT 
TGCATTTGTT TTAATTAAGC GAGAAATTGC AATCCATTCC TCAGAGCTCT 
TGACTTGGAA TCACAATAAA ATTAAATTGA TTTG

Gene models with high sequence identity

AT1G76690.1 [+] show detail - 12-oxophytodienoate reductase 2
E-value: 6e-139; Score: 491.00

InterPro Scan Digest

Program	Description	E-value
superfamily	FMN-linked oxidoreductases	3.2e-127
HMMPfam	NADH:flavin oxidoreductase/NADH oxidase, N-terminal	3.1e-83
Gene3D	Aldolase-type TIM barrel	1.2e-115
HMMPanther	12-OXOPHYTODIENOATE REDUCTASE OPR	1.1e-222
HMMPanther	NADH OXIDOREDUCTASE-RELATED	1.1e-222

See the detailed result >

AT1G76690.1

Model type: Protein coding
Short Description: 12-oxophytodienoate reductase 2
Curator Summary: Encodes one of the closely related 12-oxophytodienoic acid reductases. This enzyme is not expected to participate in jasmonic acid biosynthesis because during in vitro assays, it shows very little activity with the naturally occurring OPDA isomer. Shows activity towards 2,4,6-trinitrotoluene. Expressed predominately in root. Predicted to be a cytosolic protein.
Computational Description: 12-oxophytodienoate reductase 2 (OPR2); CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), NADH:flavin oxidoreductase/NADH oxidase, N-terminal (InterPro:IPR001155); BEST Arabidopsis thaliana protein match is: 12-oxophytodienoate reductase 1 (TAIR:AT1G76680.1); Has 13197 Blast hits to 13176 proteins in 2056 species: Archae - 127; Bacteria - 9811; Metazoa - 29; Fungi - 865; Plants - 452; Viruses - 0; Other Eukaryotes - 1913 (source: NCBI BLink).
Link: InterPro Scan - TAIR