K31033

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31033

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31033

Determination of introduced cDNA(s)

  • J023100L19 - Rice Full-length cDNAs - Original: J023100L19

    full reading [+] show sequence

    Nucleotide sequence: J023100L19 - full reading 
    GACTTCCTTC CCTTGGCGTA AAAGAAACAG AAATCTCTCG TCTCGTCGTC 
    TCCGCTCCTT GCGCCAAGAC GAGTCGCGGG TGAACAGGGG AGGGGACGCC 
    GGACGCCGAT CTCCATCTGG CGAGCAGAGC AACGGAGGGG AGGTGCGCCG 
    ATCTCCATCT GGCGAGCAGA GCAGGGGAGG GGAGGGGAGG GGATCGGGCG 
    AAATTTGTGA AATCTTTCAG TTGATGAAAT CTTAAAATCA TGGGAGAAAA 
    TACTGGCGAT AATCCAAACA TGTCAATTCT TCAAAGAATT GCCACAAGTG 
    ATGTTCCACT GGTGAAAGAG TATGGTCTGC CTGGAATTAT TGGTGCCATT 
    CTACTTGCTG TAGTTATTCC TATCATGCTT TCTTCCATAT TCAACAAAAA 
    GGGAAAGAAA AGAGCAGTAC AAGCTGATGT GGGTGGTGAG GCAGGACTTG 
    CCATGCGAAA CAGTAGATTT TCCATGTTAG TTGAAGTTCC ATGGGAGGGT 
    GCCACCACAA TGGCAGCTTT ATTTGAGATG GCTAGCAAAA AATACCCTCG 
    ACACAGGTGT CTTGGCACAA GGAAGCTAAT CAATAGAGAA TTTGTGGAGT 
    CACCTGATGG CAGGAAGTTC GAGAAATTGC ATTTAGGTGA ATATGAATGG 
    GATACCTACG CAGAAGCATT TAGTCGTGCA TGTAATTTTT CTTCTGGTCT 
    CATCAAGTTG GGTCACCAAC GAGATAGTCG TGCTGCAATA TTTGCTGATA 
    CGAGAACTGA GTGGATCATT GCTGCTCAGG GATGCTTTCG ACAAAATCTA 
    ACAGTTGTAA CTATCTATGC ATCTCTTGGC GAGGATGCAC TGGTGCATTC 
    ACTGAATGAG ACACAGGTTT CAACTCTGAT ATGTGATTCA AAGCAACTTA 
    AGAAGCTACC TGCAATCAGT TCCAAGTTGC AGAGTGTTAA GCATGTTATC 
    TATATTGAGG ATGAACCTGT TGAGGCTGAG GTACTCAATC AAATGAAGCA 
    CTGGACAACA TTTTCTTTTG GTGAAGTTGA AGAATTGGGT AAAACATCAC 
    ATACAGATGC AAGATTACCT TCAAGCACTG ATACTGCAGT TATTATGTAT 
    ACAAGTGGAA GTACGGGTCT TCCCAAGGGT GTAATGATTA CACATGGAAA 
    TATGGTGGCC ACAACTGCAG CTGTCATGAC AATCATACCA AAACTTGGCA 
    CAGGGGATGT TTATCTGGCA TACCTTCCGC TGGCTCATGT CTTTGAACTA 
    GCAGCAGAGA CTGTCATGTT AGCTTCTGGT GCTGCCATTG GATATGGCTC 
    AGCTCTGACT ATGACAGATA CATCAAATAA AATAAAGAAG GGGACAAAAG 
    GAGATGTTTC TGCACTGAAG CCTACTCTTA TGATTTCAGT CCCTGCAATT 
    TTAGACCGCA TCAGAGATGC CGTGTTTAAA AAGGTTGGTG AGAAGGGTGG 
    TCTGACCAAA AAGCTGTTTG ACATTGCCTA TAAACGAAAT CTTGGAGCTA 
    TTGAAGGAAG TTGGTTTGGA TCCTGGGCAC CAGAGAGGAT GATATGGGAT 
    AACCTCATTT TTAAACCAAT ACGTTCCATG CTTGGTGGGC GCATTAGATT 
    TGTTCTTTGT GGTGGTGCAC CTCTATCGAG TGACACACAA AGATTTATGA 
    ATATATGTCT TGGTGTACCA GTAGGTCAAG GCTATGGATT GACAGAGACT 
    TGTGCTGGAG CAGCCTTTTC TGAATGGGAT GACACAAGTG TGGGCCGTGT 
    TGGTCCACCC CTTCCTTGTT GCTATGTCAA GCTCGTTTCA TGGGAAGAAG 
    GTGGCTATAA AATTTCTGAT TCTCCAATGC CCCGAGGAGA GGTTGTAGTT 
    GGTGGATACA GCATAACAAA AGGTTATTTC AACAATGAAG CAAAAACAAA 
    TGAGGTCTAC AAGGTAGATG AGAGGGGAAT GCGCTGGTTT TACACTGGAG 
    ATATTGGCCA GTTCCATCCT GATGGGTGCC TCGAGATCAT TGATAGAAAA 
    AAGGATATAG TAAAACTTCA GCATGGAGAA TATGTGTCTC TTGGCAAGGT 
    TGAATCAGCT CTAACTACTA GCAATTATGT GGACAGCATA ATGGTTTATG 
    CTGATCCATT TCATAATTAT TGTGTGGCGT TGGTAGTGCC GGCGCACCAG 
    GCCTTAGAGC AGTGGGCTCA AAATTCTGGG ATTAACTACA AAAACTTTGA 
    TGAGCTGTGC CACAATGATC AAGCCATTAA GGAGGTCCAG CAATCTCTAT 
    CAAAGGCTGC AAAAGCAGCG AGGCTTGAGA AGTTTGAAAT ACCAGCAAAG 
    ATTGTGTTGC TGCCTGAACC ATGGACTCCA GAGTCTGGGC TGGTGACAGC 
    TGCACTTAAA CTGAAGAGGG AGCAGATAAA GACCAAATTC AAAGATGATC 
    TGGGCAAGCT CTATCACTGA ATCAGCTATG ATGTCTGCTG GGGCACCATT 
    ATGGATGAAA TAATCTATGT TCTTTTGCTA TTTGTCATCC GCAAGGCTCT 
    CTTGACGGGA TGACCTGCTC ACTAAATTCA GCGTGTAGTC AATACCCCGA 
    ATGCTAGACC CGGGAAACGA CTGGGACGTG GAGCAGTGTA ATTATGGGAT 
    TTCTCCGTGC CTTGTTAGGA TAATAAATTT CTCAAGACCG GATTAACCTG 
    CTTCTGTTCT TCTGGTTAAT AATATATAGT TGCATGTTCC TTTTCTTAGC 
    TTGGGATGGT TGTTTCGTAC TATGATGATT AACAAAAGCT GTTTCCAC

    Gene models with high sequence identity

    1. AT2G04350.2 [+] show detail - AMP-dependent synthetase and ligase family protein
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Acetyl-CoA synthetase-like 8.7e-125
    HMMPfam AMP-dependent synthetase/ligase 1.6e-110
    Gene3D no description 1.5e-33
    Gene3D no description 0.00025
    Gene3D no description 3e-13
    PatternScan AMP-binding, conserved site 0
    HMMPanther LONG-CHAIN-FATTY-ACID COA LIGASE 3.1e-259
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 3.1e-259

    See the detailed result >

    AT2G04350.2

    Model type
    Protein coding
    Short Description
    AMP-dependent synthetase and ligase family protein
    Computational Description
    long-chain acyl-CoA synthetase 8 (LACS8); FUNCTIONS IN: long-chain fatty acid-CoA ligase activity, catalytic activity; INVOLVED IN: fatty acid biosynthetic process, metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: long chain acyl-CoA synthetase 9 (TAIR:AT1G77590.1); Has 68120 Blast hits to 56487 proteins in 3592 species: Archae - 1085; Bacteria - 46156; Metazoa - 2682; Fungi - 3484; Plants - 2089; Viruses - 2; Other Eukaryotes - 12622 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR