K32413

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32413

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32413

Determination of introduced cDNA(s)

  • J013064K20 - Rice Full-length cDNAs - Original: J013064K20

    full reading [+] show sequence

    Nucleotide sequence: J013064K20 - full reading 
    GGGTCTCCAC TTGCCCCCCT CACCCTCACC CTCACCCTCT CCCCCTCGCG 
    CCGCCGGGGC GACCAGGGGC GGCTTCTCCC CGTTAGCTTT CCCGCATCGG 
    CGGGGCCCGC CGCCCCCCCG CCCGCCATGC GCGCCCTTCG CTCCCGCTTC 
    CTTCCGTGGT TACAATTTGC TGAGGGATCC TCGCTACAAC AAGGGGCTTG 
    CCTTCAACGA AAGGGAGCGC GAAACGCACT ACCTCCGTGG GCTTCTGCCA 
    CCAGCAATCG TATCCCAGGA GCTCCAGGAA AGGAAAATCA TGCATAACAT 
    CCGACAATAC CAGCTGCCTC TGCAGAAATA CATGGCTATG ATGGACCTCC 
    AGGAGGGGAA TGAGAGGCTT TTCTACAAGC TCCTCATTGA CAATGTTGAG 
    GAGCTACTTC CTGTCGTTTA CACTCCAACA GTTGGTGAGG CCTGCCAAAA 
    GTATGGATCC ATATTTAGCC GTCCTCAGGG TCTTTATATC AGCTTGAAGG 
    AGAAGGGAAA GATCCTTGAG GTGCTAAAAA ACTGGCCAGA GAGGAGCATT 
    CAGGTTATCG TTGTTACTGA TGGTGAGCGA ATTTTGGGGC TTGGAGATCT 
    TGGCTGCCAG GGAATGGGGA TCCCTGTTGG TAAGCTTGCC CTTTACACTG 
    CTCTAGGAGG AGTGCGTCCA TCTGCTTGTT TGCCAATCAC ATTGGATGTT 
    GGTACAAATA ACGAGGCGCT GCTGAATGAT GAGTTCTACA TTGGCTTAAG 
    ACAAAAGAGG GCCACTGCTC AGGAGTATGC TGATTTTCTT CATGAATTCA 
    TGACTGCCGT GAAACAAAAC TATGGGGAGA AAGTACTCAT TCAGTTTGAG 
    GATTTTGCAA ACCATAATGC ATTTGAACTC CTTGCAAAAT ATGGAACAAC 
    TCACCTTGTA TTCAATGATG ATATTCAGGG AACAGCTTCC GTGGTCCTTT 
    CTGGGCTTGT TGCAGCACTA AAATTAGTTG GTGGTTCATT GTCGGAGCAC 
    AGTTACCTGT TCCTGGGAGC TGGAGAGGCT GGGACAGGCA TTGCAGAACT 
    TATAGCTCTC GAGATATCAA GACAGACCAA AGCACCAATT GAGGAGTGCC 
    GCAAGAAAAT ATGGCTTGTT GATTCAAAGG GCTTGATTGT CAGTTCACGA 
    AAGGAGACCC TTCAACACTT CAAGAAACCA TGGGCCCATG AGCATGAACC 
    TGTTGGAAAT CTCTTAGATG CAGTCAAAAC TATCAAACCA ACAGTATTGA 
    TTGGCACATC TGGAAAGGGG CAAACTTTCA CTCAGGAGGT TGTTGAAGCC 
    ATTTCCTCAT TTAATGAGAG GCCTGTCATT TTTGCCTTGT CCAACCCAAC 
    ATCTCAGTCT GAGTGCACCG CTGAACAAGC GTACACATGG AGCAAGGGCC 
    GCGCGGTGTT TGCAAGTGGA AGTCCATTTG ATCCGGTGGA GTATGATGGC 
    AAAATATACG TGCCTGGCCA GGCAAACAAC GCCTACATCT TCCCAGGGTT 
    TGGCCTTGGT GTGGTGATGT CTGGTGCAAT CCGTGTTCAT GATGACATGC 
    TTCTTGCAGC TTCGGAAGCA CTGGCTCAGC AGGTCACACA AGAGAATTTC 
    GACAAGGGCC TTACTTATCC TCCCTTCTCA AACATCAGAA AGATCTCTGC 
    CCACATTGCA GCCAACGTTG CAGCAAAGGC ATATGAACTT GGTTTGGCAA 
    GCAGGCGCCC TCGGCCAAAG GACCTGGTTA AGTATGCAGA GAGCTGCATG 
    TACAGCCCAC TTTACCGCAA CTACCGGTGA ATCTGGTCAT CCGGTATCCG 
    GATCTATTCC AGTCATGTAT ATCTATGAAC TGTTTCCTGC AAAATCTTAC 
    TACTTGTTCA GTACTGCACG CTGTAATTCT GATGAGTCTA CCGGAGTTAC 
    TTCAAGCCTT TTTTTCCCCG TGTCGTTCAT GCCTGTATTG TAATATTTCC 
    TTTTCGACGT GATGTGAAGT TCCTTGTATC CAGTTACCTT AAGCATGGGC 
    ATATGTATTT TCATCACAAG AATGTTACCG TCCTAGGGAT GGGTAATAAA 
    TGATAAATCT GGAATAAAGT TTCTACAGTT CCTTTCTCGG T

    Gene models with high sequence identity

    1. AT1G79750.1 [+] show detail - NADP-malic enzyme 4
      E-value: 0; Score: 910.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D NAD(P)-binding domain 9.20006495413801e-93
    HMMPfam Malic enzyme, N-terminal 7.99999999999993e-75
    superfamily NAD(P)-binding domain 3e-106
    HMMPfam Malic enzyme, NAD-binding 1.10000000000001e-87
    PatternScan Malic enzyme, conserved site 0
    superfamily SSF53223 1.4e-101
    HMMPanther PTHR23406 0
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    FPrintScan Malic oxidoreductase 1.69998802370953e-85
    HMMPanther PTHR23406:SF2 0

    See the detailed result >

    AT1G79750.1

    Model type
    Protein coding
    Short Description
    NADP-malic enzyme 4
    Curator Summary
    The malic enzyme (EC 1.1.1.40) encoded by AtNADP-ME4 is localized to chloroplasts. The gene is expressed throughout the whole plant and during embryogenesis and germination. A possible involvement in the fatty acid biosynthesis has been proposed.
    Computational Description
    NADP-malic enzyme 4 (NADP-ME4); CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NADP-malic enzyme 3 (TAIR:AT5G25880.1); Has 9573 Blast hits to 9553 proteins in 2441 species: Archae - 143; Bacteria - 6332; Metazoa - 610; Fungi - 220; Plants - 473; Viruses - 0; Other Eukaryotes - 1795 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR