K32830

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32830

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32830

Determination of introduced cDNA(s)

  • J033130D23 - Rice Full-length cDNAs - Original: J033130D23

    full reading [+] show sequence

    Nucleotide sequence: J033130D23 - full reading 
    GAAACCCTAA TCCCCCTCCA AAACCCTACA CCCACCCCCT CACCTGCGGC 
    GGCTGCGGCG GCGGCGGAGA GCAACATCGT CGAGTCGACG CCATGAGGCC 
    ACCGCGAGGG AGGGGATTCG GGAGGGGAGG GAGAGGCGAT GGCGGCGGCC 
    GCAGTGGCGG CGGTGGGAGG GGATTCGGGA GGGGCGGGGA CAGCGGAGGG 
    AGAGGAGGCC GTGGGAGGGG AGGTGGCAGG ACGCCAAGGG GCCGTGGCGG 
    CGGCCGCGGT GGCGGGAGGG GTGGCATGAA GGGCGGGAGC AAGGTGGTCG 
    TGGTGCCGCA CAAGCACAAC GGCGTCTTCA TCGCCAAGGC CAAGGAGGAC 
    GCGCTCTGCA CCAAGAACAT GGTCCCCGGC GAGTCCGTCT ACGGAGAGAA 
    GCGTATCTCA GTCCAGAATG AGGATGGAAC TAAGGTTGAA TACAGGGTGT 
    GGAACCCCTT CCGTTCTAAG CTGGCTGCTG CTGTGCTTGG TGGTGTCGAC 
    AACATCTGGA TTGCTCCTGG TACTCGTGTG TTATATCTCG GTGCTGCCTC 
    AGGAACAACA GTGTCTCATG TGTCCGATAT TGTTGGACCG ACTGGTTTGG 
    TCTACGCTGT TGAGTTCTCG CACAGGAGTG GTAGGGATCT TGTTAACATG 
    GCCAAAAAGA GGACTAATGT GATCCCCATC ATTGAGGATG CTAGGCACCC 
    AGCAAGGTAC CGGATGTTGG TTGGCATGGT TGATGTTATC TTCTCTGATG 
    TTGCCCAGCC AGATCAGGGG CTTTAGCAAA TGAGGAACTT ACTCGGATTC 
    CCCTTCAGGA CATCAAATTG GATGATGCAA ATTCGAGTTT CCAATGCGCC 
    GTGCCTTCAT TGTTTCTTTC CTTTGCGTTT ATGGGTATTC TGAATAAAAT 
    ATTTTGACTG TGCTAGCAAT TTGCCTTATG CCTGTTTTAT TCTCTCGTTA 
    GTTGTAAAAC CTTCAGTATC GAAGTTGATG CGTGCCTTAT TGGTGGCCAA 
    TAGTCATGTT TTTGTCCATA TTCATTTCCT TGGCATGATC TTGTTTATTT 
    TCTTGACTAC ATCAGCTGTG GTAGGAATTT ATAGGTTTCC TGGTATAGCG 
    TTCTCTCCTA TTGTTATTAT GTTTTATGCC TCTTTGATAG TGCTAGTTAG 
    GACCAGATTG TTATGTTTTT GCTTTGTAAG GAGTAATGAA TACTGTGGAG 
    ATAAAAGTTT ACGGTTCATA TTTCTGTACT GAATAAACAT ACTTGCAGTA 
    GTTTCTATAA GGTTGCTGCT CGAGTTAAGA GATAATTTGT ATCCCATAAA 
    ATTACCTTTA TGGAATCTTC CTTGGCATGT CCCTGTGTTA AAAATCGTGA 
    AGGTTCTCAC AGAATTCAGT TTTGAAACCG TTTGCAATTT TAATCTTCTA 
    CTACCCTGCA GGCTAGGATC TTAGCCCTCA ACGCATCCTA CTTCCTGAAG 
    AATGGTGGGC ATTTTGTCAT TTCAATCAAG GCGAACTGTA TCGACTCCAC 
    CATGCCAGCT GAGGCTGTGT TCGCTAGCGA GGTTGAGAAG CTGAAGGCGG 
    ATCAGTTCAA GCCGTCGGAG CAGGTGACCC TGGAGCCCTT CGAGCGTGAC 
    CATGCCTGCG TCGTTGGTGG CTACAGGATG CCCAAGAAGC AAAAGGGCAC 
    ATCTTAATTT AAGGATACGG CCATCTGCGG TCAAAGCTCA AATGTACTAG 
    TAGAACTTTT TATAAGCGTT AGCGTTTTTG CTTAAGCTAT TTTAATTAGT 
    TCATGCTGCT GCTGGTTTTA CAGCAATCCA TGTGTACCGC GAACAGAGAA 
    GTTGAAATTG GTTGAATCGA CGCT

    Gene models with high sequence identity

    1. AT5G52470.2 [+] show detail - fibrillarin 1
      E-value: 2e-82; Score: 303.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily SSF53335 1.2000011745814e-12
    HMMPfam Fibrillarin 1.3e-25
    FPrintScan Fibrillarin 2.10000267834031e-22
    FPrintScan Fibrillarin 2.10000267834031e-22
    Gene3D G3DSA:3.40.50.150 5.89998809405682e-21
    HMMPanther Fibrillarin 6.20001486442682e-46
    PatternScan Fibrillarin, conserved site 0
    superfamily S-adenosyl-L-methionine-dependent methyltrans 6.2e-37
    HMMPfam Fibrillarin 1.4e-79
    HMMPIR Fibrillarin 5.3e-106
    HMMPanther Fibrillarin 1.4e-115
    Gene3D no description 1.6e-05
    Gene3D no description 3.7e-49
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan Fibrillarin 6.8e-64
    FPrintScan PRICHEXTENSN 7.8e-10
    FPrintScan PRICHEXTENSN 7.8e-10
    FPrintScan PRICHEXTENSN 7.8e-10

    See the detailed result >

    AT5G52470.2

    Model type
    Protein coding
    Short Description
    fibrillarin 1
    Curator Summary
    encodes a fibrillarin, a key nucleolar protein in eukaryotes which associates with box C/D small nucleolar RNAs (snoRNAs) directing 2'-O-ribose methylation of the rRNA. This gene also encodes a novel box C/D snoRNA, U60.1f in its fifth intron that accumulates in seedlings and that their targeted residue on the 25 S rRNA is methylated.
    Computational Description
    fibrillarin 1 (FIB1); FUNCTIONS IN: snoRNA binding; INVOLVED IN: RNA methylation, rRNA processing; LOCATED IN: nucleolus, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fibrillarin (InterPro:IPR000692); BEST Arabidopsis thaliana protein match is: fibrillarin 2 (TAIR:AT4G25630.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR