K36542

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K36542

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K36542

Determination of introduced cDNA(s)

  • J033124L10 - Rice Full-length cDNAs - Original: J033124L10

    full reading [+] show sequence

    Nucleotide sequence: J033124L10 - full reading 
    GGTCTTCTTC CTTCCTCGGC CCGGCCTCGC TCGCTGCCTG CCAGCTGCAG 
    CTGCAACTCT CCATCCATCG ATCGATCTCC TCCTACTCCC CCGCCGCCGC 
    CCCGTCCAAG ACAAGCCAAG CCAGCCACCA CTCCCTGCAT GCCATGGCCT 
    CACGGAGGCC AACGCACCGC CACCATACCG AGGCTCCAGA TCCTGGTGGA 
    AGAGGGAGAG GGAGAGGCCG TGCCGCCCGA TATGCTCAGC CTCAGCCTCA 
    GCCTCAGCAG CAGCAGCAGC AGCAAGGACG AGGATGCCGT GCTAGAGGTG 
    CCTCGCCGCC GCCGCCGCCG CAGCAGCAGC AGCAGCAACA GCCAAGGTCT 
    ACTCCTACTC GTGCTACCAC TGTCACCGTG GCTTCCTCTT CATCTACCAC 
    GGCCACCGCA TCATCAAGCC CTCTAGCTCC CGAGCTGCGC CAAGCAATAA 
    TGGAAGCTCC CCGTCCCAGT GAGCTCGCTC AACCCTCACC CACTCCGCCT 
    CAGGAACAAC CTGTGGATGC CGCTACCACG ACGCCGCACC ATATTCCATC 
    CTCAAGCAAG TCAATTAGGT TTCCTTTGCG CCCGGGGAAG GGTACCATTG 
    GCACCAGGTG CATGGTCAAG GCCAATCATT TCTTCGCTCA CCTGCCCAAC 
    AAGGATCTTC ATCACTACGA TGTCTCCATC ACTCCAGAAG TTACATCACG 
    CATTGTTAAC CGAGCTGTGA TCAAGGAGCT GGTAAATCTG TACAAGGCAT 
    CTTATCTGGG TGGGAGGCTG CCTGCATATG ATGGTAGAAA GAGCTTATAC 
    ACGGCTGGCC CATTGCCGTT TACTTCACAG GAGTTTCAGA TCACTTTACT 
    TGACGACGAT GATGGTTCTG GTTCTGAGAG GCGACAGAGG ACTTTCCGAG 
    TAGTAATCAA GTTCGCCGCA CGGGCCGACC TTCACCGGCT TGAGTTGTTT 
    TTAGCTGGGA GGCACGCAGA AGCTCCACAG GAGGCACTGC AAGTTCTTGA 
    TATTGTGCTG CGAGAGCTAC CATCAGCAAG ATATGCGCCA TTTGGACGTT 
    CCTTCTTTTC GGCTTACTTG GGTAGGAGGC AACCCCTTGG TGAGGGATTG 
    GAAAGCTGGC GTGGATTTTA CCAGAGCATT CGGCCTACTC AGATGGGCTT 
    ATCACTGAAT ATTGATATGT CAGCTACAGC TTTCATTGAG CCGTTACCTG 
    TTATTGATTT TGTTGCACAA CTATTGAATT CTGACATCCA TTCAAGGCCG 
    CTTTCTGATG CCGAACGTGT TAAGATCAAG AAAGCCTTGA GAGGAGTAAA 
    GGTGGAAGTT ACCCACCGTG GCAACATGAG GCGGAAGTAC CGGATATCTG 
    GTTTGACAAT TCAGCCAACT CGTGAGCTAA CTTTTCCTGT TGATGAAGGA 
    GGCACAGTGA AGTCAGTTGT ACAGTACTTT CAAGAGACAT ATGGCTTTGC 
    CATCCAACAC ACCTACCTTC CATGCCTTAC AGTTCAGCGA TTAAATTACC 
    TGCCTATGGA GGTCTGCAAA ATAGTGGAAG GACAGAGATA CTCCAAGAGA 
    TTAAATCAGA ATCAGATAAG AGCTCTTTTG GAGGAGACAT GCCAACACCC 
    ACGTGATCGG GAGCGTGATA TTATTAAGAT GGTTAAACAT AACGCTTATC 
    AGGATGATCC TTACGCAAAA GAGTTTGGCA TTAAGATAAG TGATCGTCTG 
    GCATCAGTAG AGGCACGAAT TTTACCGGCT CCACGGCTTA AGTACAATGA 
    GACTGGTCGA GAGAAGGATT GCTTACCAAG AGTTGGTCAA TGGAATATGA 
    TGAACAAGAA AATGGTAAAT GGTGGTAAAG TCAGAAGCTG GATGTGCGTC 
    AATTTTGCCC GTAATGTGCA GGAAAGTGTT GTTCGTGGGT TCTGCCATGA 
    ACTTGCTCTG ATGTGTCAAG CCTCAGGAAT GGATTTCGCT CCAGAACCTA 
    TCCTTCCACC ACTAAATGCA CATCCAGATC AAGTGGAGCG TGCTCTAAAA 
    GCTAGGTATC ATGATGCAAT GAACGTTCTT GGACCCCAGC GCAGGGAACT 
    TGATTTGCTT ATTGGGATAC TACCTGATAA CAATGGCTCG CTTTATGGTG 
    ATTTGAAGCG CGTGTGTGAA ATAGATCTTG GAATAGTTTC ACAATGCTGT 
    TGCACGAAGC AGGTGTTCAA AATGAACAAA CAAATTCTTG CAAATCTTGC 
    TCTGAAGATA AATGTCAAGG TTGGGGGCAG GAACACTGTG CTGGTGGATG 
    CTGTGTCAAG GCGTATTCCT CTGGTAACCG ACAGACCTAC AATTATATTT 
    GGTGCTGATG TTACCCATCC TCATCCTGGA GAGGACAGCA GTCCCTCAAT 
    TGCTGCTGTT GTAGCCTCCC AAGATTGGCC TGAGGTGACA AAGTATGCTG 
    GGTTGGTTTC TGCTCAAGCC CACCGACAAG AGCTGATAGA AGATCTATAT 
    AAAATCTGGC AGGATCCACA GAGAGGAACA GTTAGTGGTG GCATGATCCG 
    TGAGCTGCTT ATATCCTTCA AAAGATCAAC TGGTGAGAAG CCCCAGCGAA 
    TAATATTTTA CAGGGATGGC GTTAGTGAAG GCCAATTTTA CCAAGTTCTA 
    CTTTATGAAT TGAATGCAAT CCGAAAAGCA TGTGCCTCCC TGGAGACAAA 
    TTACCAACCA AAGGTGACTT TCATTGTGGT TCAGAAACGT CACCACACAA 
    GATTATTTGC ACATAATCAC AACGATCAGA ACTCAGTTGA CAGGAGCGGG 
    AACATACTCC CTGGTACGGT TGTAGATTCA AAGATCCGTC ATCCAACTGA 
    GTTTGACTTC TACCTGTGTA GCCATGCTGG CATTAAGGGT ACTAGTCGTC 
    CAGCTCATTA TCATGTCTTG TGGGATGAAA ACAACTTCAC AGCTGATGCA 
    TTGCAGATTC TTACCAACAA CCTTTGCTAC ACCTATGCAA GGTGCACTCG 
    CTCTGTATCA ATTGTTCCAC CTGCTTATTA TGCTCATCTG GCTGCCTTCC 
    GTGCTCGTTT CTATATGGAA CCAGATACAT CTGACAGCAG CTCTGTCGTT 
    AGTGGGCCTG GTGTACGTGG GCCACTTTCT GGCTCATCAA CATCACGTAC 
    TCGGGCCCCT GGTGGTGCAG CTGTTAAGCC ACTTCCTGCT CTGAAGGATA 
    GTGTGAAGAG GGTCATGTTC TACTGCTGAA GCTAGGGCCT ACATAGCTAA 
    AGCTCTTCGT TTCTTGGCAA CCTGCCTATG ATGGTTGTAA TTATGTGTCA 
    AAAAATCCCA TAATAATCTG CCAGCTGCTA TCTTCTCCAT TGTACTATGC 
    TGGTCATGTT TGCCAAAGTT ACCCTATATG TATGTATATT ATGCTATTGT 
    TTTTTTTTTT TTTGCGGGGA CTATTGTTAT CATGTATTAT GTTTTACTGT 
    CATGTCTGTA GTTATGATTC GAGGAGTTTG CG

    Gene models with high sequence identity

    1. AT1G48410.1 [+] show detail - Stabilizer of iron transporter SufD / Polynucleotidyl transferase
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    superfamily PAZ domain 2.2e-56
    superfamily Polynucleotidyl transferase, ribonuclease H fold 5.3e-25
    ProfileScan Argonaute/Dicer protein, PAZ 31.305
    ProfileScan Stem cell self-renewal protein Piwi 57.72
    HMMPfam Stem cell self-renewal protein Piwi 1.6e-109
    HMMPfam Argonaute/Dicer protein, PAZ 3.4e-31
    HMMPfam Domain of unknown function DUF1785 1.4e-22
    HMMPanther EUKARYOTIC TRANSLATION INITIATION FACTOR 2C 8e-284
    Gene3D no description 7e-41

    See the detailed result >

    AT1G48410.1

    Model type
    Protein coding
    Short Description
    Stabilizer of iron transporter SufD / Polynucleotidyl transferase
    Curator Summary
    Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.
    Computational Description
    ARGONAUTE 1 (AGO1); FUNCTIONS IN: protein binding, endoribonuclease activity, siRNA binding, miRNA binding; INVOLVED IN: in 11 processes; LOCATED IN: cytosol, nucleus, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Domain of unknown function DUF1785 (InterPro:IPR014811), Stem cell self-renewal protein Piwi (InterPro:IPR003165), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Polynucleotidyl transferase, ribonuclease H fold (InterPro:IPR012337); BEST Arabidopsis thaliana protein match is: Stabilizer of iron transporter SufD / Polynucleotidyl transferase (TAIR:AT5G43810.2); Has 29249 Blast hits to 15917 proteins in 1190 species: Archae - 22; Bacteria - 5845; Metazoa - 12576; Fungi - 2412; Plants - 4639; Viruses - 404; Other Eukaryotes - 3351 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR