R90262

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: R90262

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90262

Determination of introduced cDNA(s)

  • J013105J05 - Rice Full-length cDNAs - Original: J013105J05

    full reading [+] show sequence

    Nucleotide sequence: J013105J05 - full reading 
    GTCTCCCTGC TGCTGGTACT GTGCGTGGGC CGGAGGGCGA GAGTGAAGCT 
    TGAGCCGTCT CGCCGCGGGC GCTGCTGCTG CTGCTGCTGC TCGCGTCCGA 
    TTCCGACGAG AGGCTGGGAA CTTCAAAGCT GAAAAGGACT AATGGAGGGT 
    GGAGGCGCAA AAGATGGTTC TGCAGCGGCA GCGCAAACAA GGGGGAGTGG 
    GGATGATGGT TCCCACAAGC CGCTGCCGCC TTGCTGTCTC AAGGCGAAGG 
    CTGCGGCAGC AGAGTCTGAG GCAAAATGCC ATGCCACAGT GGTGTCAGGG 
    TGGTTCACGG AACCCCGCTC GCACTCTGGT AAAACAAGCA AAGTGCAGTA 
    CTTCAACAAT CCAATGTGGC CTGGAGAAGC TCATTCTCTG AAAGTAGAGA 
    ATATTCTGTA TCAAGGGAAA TCACCATACC AAGAAATCTT AGTTTTTGAG 
    TCCTCAACCT ATGGGAATGT TCTGGTGCTT GATGGTATTG TGCAGCTGAC 
    CGAAAAGGAT GAATGTGCAT ACCAGGAAAT GGTTACTCAC CTTCCACTGT 
    GCTCGATTCC ATCCCCCAAA AGTGTTTTGG TTGTCGGAGG TGGTGATGGT 
    GGTGTGCTAC GAGAAATAGC CAGACATGCC TCAGTGGAGA ACATCGATAT 
    ATGTGAAATA GACCAGCTAG TTATTGATGT CTGTAAAGAT TTCTTCCCAC 
    AACTATCTGT TGGATTCAAA GATCCCCGTG TTCAACTTCA TGTTGGTGAC 
    GCTGTGGATT TCTTGAGAAA TGCCCCTGAA GGGAAATATG ATGCTATCAT 
    TGTTGATTCA TCTGACCCAA TTGGGCCAGC TCAGGAGCTT GTGGAGAAGC 
    CCTTTTTTCA GACAATTGCT AGAGCTCTAA AGCCTGGTGG AGTTCTATGC 
    AATCAAGCTG AGAGTATGTG GCTGCACACA CATCTAATTC AAGATATGCT 
    TTCTATCTGT CGTGAAACAT TCAAGGGAGC TGTGCATTAT GCCTGGACAA 
    GCGTTCCTAC CTATCCTAGT GGTGTCATTG GTTTTTTGCT ATGTGCGAAA 
    GAAGGTCCAG CAGTGAACTT CCTTTCTCCT GTGAATCCAA TTGAGAAACT 
    GGAAGGAGCT ATGGAAGCTG GAAGGGAAAT CAGATTTTAC AATTCAGAGG 
    TTCATCGGGC TGCTTTTGTT CTGCCAACAT TTGTAAGGAG AGAACTGGAA 
    TCACACAATA CTTGTGCTGA AAAGGATAAA TCAGAAACCA AGCCAGTCGC 
    GAAACCAAAG AAGATGAAAA TAATGCCGAA TAGTGCCATT CCGACTGCTT 
    CCTAGATTCG GGCGGCCATA TGCGACAACC ATGTTCGACG TCCTTAGCTA 
    CAGTATATGC CAACTATAAG AGGAGAATGT CGCTGTAACT CTTCTGTCTT 
    AAGTATGGTA TGTTATGCCT GACTGAATAA AAGGGAGGAA CAGATGAGAT 
    GTGAACCAAA ATAATGTTCT TCCTGATCCG TGAGCACTGT TGTAGTGTTG 
    CCTGTTGTAC AAATGTATAT TGTCACAGCA CTGATGTGTT TATTATATGG 
    ATGTGGAGTG AATCGCATGG ATAATGTGCC TTTTTATCCC CTT

    Gene models with high sequence identity

    1. AT5G53120.5 [+] show detail - spermidine synthase 3
      E-value: 3e-134; Score: 475.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Spermine synthase 101.41
    HMMPfam Spermine synthase 2.6e-89
    HAMAP SPEE_MF_00198 32.074
    Gene3D no description 1.8e-09
    Gene3D no description 1.1e-80
    superfamily S-adenosyl-L-methionine-dependent methyltrans 5.9e-92
    PatternScan Spermine synthase 0
    HMMPanther SPERMIDINE SYNTHASE 3.7e-206
    HMMPanther Spermine synthase 3.7e-206
    HMMTigr Spermine synthase 7.4e-115

    See the detailed result >

    AT5G53120.5

    Model type
    Protein coding
    Short Description
    spermidine synthase 3
    Curator Summary
    encodes a novel spermine synthase and is a paralog of previously characterized spermidine synthases, SPDS1 and SPDS2. SPDS3 forms heterodimers with SDPS2, which in turn forms heterodimers with SDPS1 in vivo. The gene does not complement speDelta3 deficiency of spermidine synthase in yeast but DOES complement speDelta4 deficiency.
    Computational Description
    spermidine synthase 3 (SPDS3); CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: spermidine synthase 1 (TAIR:AT1G23820.1); Has 5367 Blast hits to 5364 proteins in 1535 species: Archae - 174; Bacteria - 2998; Metazoa - 341; Fungi - 210; Plants - 426; Viruses - 0; Other Eukaryotes - 1218 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR