R90377

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: R90377

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90377

Determination of introduced cDNA(s)

  • J033097H10 - Rice Full-length cDNAs - Original: J033097H10

    full reading [+] show sequence

    Nucleotide sequence: J033097H10 - full reading 
    GGCTCACAAA TCTCCGCTTC GTCCCCCACC CACCCACCAC TCCACTCCCC 
    GCAGTTGGTG GCCCGCGGCG GCGCAAGCCC TAGCGTCCAC GCGAAGCAAA 
    CCCCCCAAAA CCCTAGCTCC GCCGCCGTCG GCGGCGCCCG AGCTGCAACC 
    CCCGCCACGA CGCCATCCGG GTGGCTAGCC TAAGCCCCTA ACCAAACCCT 
    AGCGACCGGC GCCGCAGTCG GGGGGAGAGA TGCCGCGGGA GATCATCACG 
    ATACAGGTGG GGCAATGCGG GAACCAGATC GGGATGGAGT TCTGGAAGCA 
    GCTCTGCCTC GAGCACGGCA TCGGCAAGGA CGGCCTCCTC GAGGACTTCG 
    CCACCCAGGG AGGAGATAGG AAGGATGTTT TCTTCTACCA GGCTGATGAC 
    CAGCATTACA TACCGAGGGC TCTTCTTGTG GATCTAGAGC CGAGAGTGAT 
    CAACGGAATA CAGAACAGTG AGTACAGGAA TCTATACAAT CATGAGAATA 
    TATTTGTGGC TGAGCATGGA GGTGGTGCTG GGAACAACTG GGCTAGTGGA 
    TATCACCAGG GTGAGCAAGT TGTTGATGAT ATCATGGATA TGATAGACAG 
    AGAAGCAGAT GGAAGTGATA GCCTTGAGGG TTTTGTGTTA TGCCACTCAA 
    TTGCTGGTGG AACTGGTTCA GGTATGGGTT CTTACCTGTT GGAGACTCTG 
    AATGACCGAT ACAGCAAAAA GCTTGTCCAG ACATACAGTG TTTTCCCAAA 
    TCAGATGGAA ACAAGTGATG TTGTTGTCCA GCCATATAAT TCGCTTTTAA 
    CTCTGAAGCG GCTGACACTG AATGCTGACT GTGTGGTTGT TCTTGACAAT 
    ACGGCCCTTA ATAGGATTGC TGTGGAACGT CTTCATTTAG CAAATCCTAC 
    TTTTGCACAA ACAAACTCTT TGGTCTCAAC AGTTATGTCT GCAAGCACAA 
    CTACTCTGAG GTATCCTGGA TATATGAACA ATGACCTGGT TGGCCTTCTT 
    GCCTCTTTGA TCCCTACTCC AAGGTGTCAT TTTCTGATGA CAGGTTACAC 
    TCCATTGACT GTTGAACGAC AGGTTAACAT GATACGCAAG ACAACAGTAT 
    TAGATGTTAT GAGGCGACTT CTGCAGACCA AAAATATAAT GGTGTCATCA 
    TATGCTCGAA ATAAGGAAGC TAGTCAGGCT AAATACATCT CCATACTTAA 
    CATCATTCAG GGAGAGGTGG ATCCTACACA GGTCCATGAG AGCCTACAAA 
    GGATACGTGA AAGGAAGCTT GTTAACTTCA TTGAGTGGGG ACCTGCAAGC 
    ATTCAGGTTG CCTTATCAAG AAAATCTCCA TATGTACAAA CCACGCATAG 
    GGTTAGTGGA TTGATGCTGG CGAATCATAC TAGTATCCGT CACTTATTCA 
    GCAAATGCCT GGGACAGTAT GAAAAGCTAA GGAAAAAGCA AGCTTTTCTT 
    GACAACTACA GAAAGTTCCC AATGTTTGAG GACAATGATC TCTCTGAATT 
    TGATGAATCT CGAGAAATAA TAGAGAGCTT AGTTGATGAG TACAAGGCTT 
    GCGAATCACC AGACTACATC AAATGGGGGA TGGAGGATGC TGGAGAGGCA 
    AACGTTGCGG CCGCACTTGA TTCGAAGTTA GTGGTGTAAA TATGAAGGTC 
    CTACAAGAAT GCAACTCTAA TTGCCTGATG GTCATCAGTT CGATGGCAGC 
    TGCTCGACTC GTTCCCACTC TAGTGCTAGA TGCGTTTCCT CAGCATTCTT 
    CAAGTTTGCT TCTCGTGTTA GTGTTACAGC CTGTGCGACA TGAGTGTATA 
    TCTTGACCCA CTTCGAACTG GCCGTAGTTT GTGCTGTTCC GTTTTGGGTT 
    GCAATCCCTT CTGGGATGCT TTGTATGTGT GGATGCGTGT GATGTACTGA 
    TACTGATGGG TTTTGTGCCA GAATAGCATG CAGAGCTCTA ATTGTTGGTG 
    C

    Gene models with high sequence identity

    1. AT3G61650.1 [+] show detail - gamma-tubulin
      E-value: 0; Score: 883.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Tubulin/FtsZ, GTPase domain 7.6e-75
    HMMPfam Tubulin/FtsZ, 2-layer sandwich domain 7.3e-48
    HMMPanther TUBULIN GAMMA CHAIN 1.4e-223
    HMMPanther Tubulin 1.4e-223
    Gene3D Tubulin/FtsZ, GTPase domain 3.1e-102
    Gene3D Tubulin/FtsZ, 2-layer sandwich domain 4e-27
    superfamily Tubulin/FtsZ, N-terminal 8.3e-92
    superfamily Tubulin/FtsZ, C-terminal 1.3e-62
    PatternScan Tubulin, conserved site 0
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Gamma tubulin 9.5e-87
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    FPrintScan Tubulin 6.6e-65
    HMMSmart no description 2.1e-58
    HMMSmart Tubulin/FtsZ, 2-layer sandwich domain 1.9e-27

    See the detailed result >

    AT3G61650.1

    Model type
    Protein coding
    Short Description
    gamma-tubulin
    Curator Summary
    Required for centrosomal and noncentrosomal microtubule nucleation. Involved in specification of cell identity, such as stomatal patterning. Constitutively expressed throughout plant.
    Computational Description
    gamma-tubulin (TUBG1); FUNCTIONS IN: structural molecule activity, GTP binding, GTPase activity; INVOLVED IN: in 9 processes; LOCATED IN: mitochondrion, microtubule, plasma membrane, cytoplasm; EXPRESSED IN: guard cell; CONTAINS InterPro DOMAIN/s: Gamma tubulin (InterPro:IPR002454), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: gamma-tubulin complex protein 2 (TAIR:AT5G05620.1); Has 22646 Blast hits to 22602 proteins in 4766 species: Archae - 19; Bacteria - 43; Metazoa - 4166; Fungi - 13770; Plants - 1412; Viruses - 0; Other Eukaryotes - 3236 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR